Recent methods for discovering novel bioactive metabolites, specifically antimicrobial agents, from marine‐associated micro‐organisms

• Published in: Letters in applied microbiology Vol. 75; no. 3; pp. 511 - 525
• Main Authors: Sedeek, A.M., Ismail, M.M., Elsayed, T.R., Ramadan, M.A.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.09.2022
• Subjects: Antiinfectives and antibacterials; antimicrobial; Antimicrobial agents; Bioactive compounds; Biological activity; Cultivation; Culture media; culture‐dependent methods; culture‐independent methods; Dilution; Genomes; in situ cultivation; marine micro‐organisms; Metabolites; Metagenomics; Microbiology; Microorganisms; Organisms; Proteomics; antimicrobial; marine micro-organisms; culture-independent methods; in situ cultivation; culture-dependent methods
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/lam.13728

Marine micro‐organisms are a promising source for novel natural compounds with many medical and biotechnological applications. Here, we demonstrate limitations and recent strategies for investigating the marine microbial community for novel bioactive metabolites, specifically those of antimicrobial potential. These strategies include culture‐dependent methods such as modifying the standard culture media, including changing the gelling agent, dissolving vehicle, media supplementation and preparation to access a broader range of bacterial diversity from marine samples. Furthermore, we discuss strategies such as in situ cultivation, dilution‐to‐extinction cultivation and long‐term incubation. We are presenting recent applications of culture‐independent methods such as genome mining, proteomics profiling and the application of metagenomics as a novel strategy for structure confirmation in the discovery of the marine micro‐organism for novel antimicrobial metabolites. We present this review as a simple guide and a helpful resource for those who seek to enter the challenging field of applied marine microbiology. Significance and Impact of the Study: Recent culture‐dependent and independent methods for the discovery of bioactive metabolites from marine microbiota.