A mixture of Lactobacillus species isolated from traditional fermented foods promote recovery from antibiotic‐induced intestinal disruption in mice

• Published in: Journal of applied microbiology Vol. 124; no. 3; pp. 842 - 854
• Main Authors: Shi, Y., Zhao, X., Zhao, J., Zhang, H., Zhai, Q., Narbad, A., Chen, W.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.03.2018
• Subjects: Ampicillin; Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - adverse effects; Anti-Bacterial Agents - pharmacology; Antibiotics; Bacteroidetes; Brassica - microbiology; Cecum - immunology; Cecum - microbiology; Colon; Colon - immunology; Colon - microbiology; Communities; Community structure; Cytokines; Cytokines - genetics; Cytokines - immunology; Digestive system; diseases; Dysbacteriosis; Ecological effects; Ecological monitoring; Endotoxins; Fermented food; Fermented Foods and Beverages - microbiology; Food; Gastrointestinal Microbiome - drug effects; Gastrointestinal tract; Humans; Ileum; Ileum - immunology; Ileum - microbiology; immunology; Inflammation; Interleukin 1; Interleukin 6; Intestinal Diseases - drug therapy; Intestinal Diseases - etiology; Intestinal Diseases - immunology; Intestinal Diseases - microbiology; intestinal microbiota; Intestinal microflora; Intestinal Mucosa - metabolism; Intestine; Intestines - microbiology; Lactic acid; Lactobacillus; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus - physiology; Lipopolysaccharides; Male; Mice; Mice, Inbred C57BL; Microbiota; Microorganisms; Monocyte chemoattractant protein 1; Permeability; Probiotics; Probiotics - administration & dosage; Proteins; Recovery; Restoration; Rodents; Species; Traditional foods; Tumor necrosis factor; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Tumor necrosis factor-α; γ-Interferon; intestinal microbiota; diseases; antibiotics; immunology; Lactobacillus
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.13687

Aims This study evaluated the antibiotic‐induced changes in microbial ecology, intestinal dysbiosis and low‐grade inflammation; and the combined effect of four different Lactobacillus species on recovery of microbiota composition and improvement of gut barrier function in mice. Methods and Results Administration of the antibiotic ampicillin for 2 weeks decreased microbial community diversity, induced caecum tumefaction and increased gut permeability in mice. Application of a probiotic cocktail of four Lactobacillus species (JUP‐Y4) modulated the microbiota community structure and promoted the abundance of potentially beneficial bacteria such as Akkermansia. Ampicillin administration led to a decline in Bacteroidetes from 46·6 ± 3·91% to 0·264 ± 0·0362%; the addition of JUP‐Y4 restored this to 41·4 ± 2·87%. This probiotic supplementation was more effective than natural restoration, where the levels of Bacteroidetes were only restored to 29·3 ± 2·07%. Interestingly, JUP‐Y4 treatment was more effective in the restoration of microbiota in faecal samples than in caecal samples. JUP‐Y4 also significantly reduced the levels of d‐lactate and endotoxin (lipopolysaccharide, LPS) in the serum of mice, and increased the expression of tight‐junction proteins while reducing the production of inflammatory cytokines (TNF‐α, IL‐6, MCP‐1, IFN‐γ and IL‐1β) in the ileum and the colon of antibiotic‐treated mice. Conclusions JUP‐Y4 not only promoted recovery from antibiotic‐induced gut dysbiosis, but also enhanced the function of the gut barrier, reduced inflammation and lowered levels of circulating endotoxin in mice. Significance and Impact of the Study Consumption of a mixture of Lactobacillus species may encourage faster recovery from antibiotic‐induced gut dysbiosis and gut microbiota‐related immune disturbance.