Sucrose nonfermenting AMPK‐related kinase (SNARK) regulates exercise‐stimulated and ischemia‐stimulated glucose transport in the heart

• Published in: Journal of cellular biochemistry Vol. 120; no. 1; pp. 685 - 696
• Main Authors: Sun, Xiang‐Lan, Lessard, Sarah J., An, Ding, Koh, Ho‐Jin, Esumi, Hiroyasu, Hirshman, Michael F., Goodyear, Laurie J.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.01.2019
• Subjects: Akt substrate of 160 kDa; AMP; Animals; Biological Transport; Cardiomyocytes; Cell Line, Tumor; Contraction; Coronary Vessels - metabolism; Fitness equipment; Gene Knockdown Techniques; Glucose; Glucose - metabolism; Glucose transport; Glycogen; Heart; HL1 cardiomyocytes; In vivo methods and tests; Insulin; Insulin - pharmacology; Ischemia; Ischemia - metabolism; Kinases; Male; Mice; Mice, Inbred ICR; Mice, Knockout; Muscle contraction; Muscles; Myocardium - metabolism; Myocytes, Cardiac - metabolism; Phosphorylation; Physical Conditioning, Animal; Protein kinase; Protein-Serine-Threonine Kinases - genetics; Proteins; Signal Transduction - drug effects; Skeletal muscle; Sucrose; Sugar; glycogen; Akt substrate of 160 kDa; insulin; HL1 cardiomyocytes
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.27425

The signaling mechanisms mediating myocardial glucose transport are not fully understood. Sucrose nonfermenting AMP‐activated protein kinase (AMPK)‐related kinase (SNARK) is an AMPK‐related protein kinase that is expressed in the heart and has been implicated in contraction‐stimulated glucose transport in mouse skeletal muscle. We first determined if SNARK is phosphorylated on Thr208, a site critical for SNARK activity. Mice were treated with exercise, ischemia, submaximal insulin, or maximal insulin. Treadmill exercise slightly, but significantly increased SNARK Thr208 phosphorylation. Ischemia also increased SNARK Thr208 phosphorylation, but there was no effect of submaximal or maximal insulin. HL1 cardiomyocytes were used to overexpress wild‐type (WT) SNARK and to knockdown endogenous SNARK. Overexpression of WT SNARK had no effect on ischemia‐stimulated glucose transport; however, SNARK knockdown significantly decreased ischemia‐stimulated glucose transport. SNARK overexpression or knockdown did not alter insulin‐stimulated glucose transport or glycogen concentrations. To study SNARK function in vivo, SNARK heterozygous knockout mice (SNARK+/−) and WT littermates performed treadmill exercise. Exercise‐stimulated glucose transport was decreased by ~50% in hearts from SNARK+/− mice. In summary, exercise and ischemia increase SNARK Thr208 phosphorylation in the heart and SNARK regulates exercise‐stimulated and ischemia‐stimulated glucose transport. SNARK is a novel mediator of insulin‐independent glucose transport in the heart. Exercise and ischemia increase sucrose nonfermenting AMP‐activated protein kinase (AMPK)‐related kinase (SNARK) Thr208 phosphorylation in the heart and SNARK regulates exercise‐stimulated and ischemia‐stimulated glucose transport. SNARK is a novel mediator of insulin‐independent glucose transport in the heart.