Tributyltin is a potent inducer of the heat shock response in human diploid fibroblasts

Submicromolar concentrations of tributyltin (TBT), a commercially used organotin compound, were found to induce the expression of several stress proteins, most notably HSP89 and HSP70, in IMR-90 human diploid fibroblasts in a time- and dose-dependent manner. This induction can be demonstrated by qua...

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Bibliographic Details
Published inJournal of cellular physiology Vol. 153; no. 3; p. 460
Main Authors Zhang, H, Liu, A Y
Format Journal Article
LanguageEnglish
Published United States 01.12.1992
Subjects
Base Sequence
Diploidy
Dose-Response Relationship, Drug
Fibroblasts - drug effects
Fibroblasts - metabolism
Fibroblasts - physiology
Gene Expression
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Humans
Molecular Sequence Data
Time Factors
Trialkyltin Compounds - pharmacology
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Summary:Submicromolar concentrations of tributyltin (TBT), a commercially used organotin compound, were found to induce the expression of several stress proteins, most notably HSP89 and HSP70, in IMR-90 human diploid fibroblasts in a time- and dose-dependent manner. This induction can be demonstrated by quantitation of 1) synthesis of the heat shock proteins (HSPs), 2) relative abundance of mRNA of hsp70, and 3) transient expression of a human hsp70 promoter driven reporter gene. TBT also increased the abundance of mRNA of heme oxygenase, whereas heat shock was without effect. Analysis of protein binding to a consensus heat shock element (HSE) by electrophoretic mobility shift assay suggests that the induction of the heat shock response by TBT was attributable to activation of the heat shock transcription factor (HSTF).
ISSN:0021-9541
DOI:10.1002/jcp.1041530304