The antiapoptotic and antioxidant effects of eugenol against cisplatin‐induced testicular damage in the experimental model

• Published in: Andrologia Vol. 51; no. 9; pp. e13353 - n/a
• Main Authors: Ekinci Akdemir, Fazile N., Yildirim, Serkan, Kandemir, Fatih M., Aksu, Emrah H., Guler, Mustafa C., Kiziltunc Ozmen, Hilal, Kucukler, Sefa, Eser, Gizem
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.10.2019
• Subjects: Animals; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - adverse effects; Antioxidants; Antioxidants - administration & dosage; Apoptosis; Apoptosis - drug effects; Chemotherapy; Cisplatin; Cisplatin - administration & dosage; Cisplatin - adverse effects; Disease Models, Animal; Enzymatic activity; Enzymes; Eugenol; Eugenol - administration & dosage; Humans; Injections, Intraperitoneal; Lipid peroxidation; Lipid Peroxidation - drug effects; Male; Oxidative stress; Oxidative Stress - drug effects; Rats; Spermatozoa - drug effects; Spermatozoa - pathology; Testes; Testicular Diseases - chemically induced; Testicular Diseases - drug therapy; Testicular Diseases - pathology; testis; Testis - drug effects; Testis - pathology; toxicity; Treatment Outcome; toxicity; eugenol; testis; cisplatin
• ISSN: 0303-4569; 1439-0272
• DOI: 10.1111/and.13353

Testicular dysfunction or damage is among the critical side effects of chemotherapeutic drugs like cisplatin. This study was mapped out to assess the possible therapeutic effect of eugenol on cisplatin‐induced testicular damage. In this experimental study, a single dose of cisplatin (15 mg/kg) was given intraperitoneally. After 72 hr of cisplatin injection, rats were sacrificed and testis tissues were removed. Tissues were examined by biochemical, histopathological and immunohistochemical methods. While tissue lipid peroxidation product and apoptotic marker levels increased, antioxidant enzyme activities of testis tissue were decreased in the cisplatin group. Additionally, histopathological damage was also determined in testis tissue. Contrary to all these results, the severity of damage in the tissue was reduced histopathologically owing to eugenol treatment. The lipid peroxidation decreased and antioxidant enzyme activities increased in the eugenol treatment group. It has been determined that eugenol has a therapeutic effect on oxidative stress and apoptosis against cisplatin‐induced testicular damage.