Non‐apoptotic cell death such as pyroptosis, autophagy, necroptosis and ferroptosis acts as partners to induce testicular cell death after scrotal hyperthermia in mice

Cell death is a biologically uncontrollable and regulated process associated with human diseases which usually occur in response to oxidative stress that activates signalling pathways in multiple forms and can therefore contribute to human diseases. Thus, the current study aims to evaluate the signa...

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Published inAndrologia Vol. 54; no. 2; pp. e14320 - n/a
Main Authors Hasani, Amirhosein, Khosravi, Amirreza, Behnam, Paria, Ramezani, Fahim, Eslami Farsani, Bahram, Aliaghaei, Abbas, Pirani, Maryam, Akaberi‐Nasrabadi, Soheila, Abdi, Shabnam, Abdollahifar, Mohammad‐Amin
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 01.03.2022
Subjects
Animals
Apoptosis
Autophagy
Caspase
Cell Death
Ferroptosis
Fever
Hyperthermia
Hyperthermia, Induced
Male
Mice
Necroptosis
Oxidative stress
Protein expression
Pyroptosis
scrotal hyperthermia
Signal transduction
Spermatogenesis
Spermatozoa
Testes
Testis
pyroptosis
autophagy
ferroptosis
scrotal hyperthermia
necroptosis
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Summary:Cell death is a biologically uncontrollable and regulated process associated with human diseases which usually occur in response to oxidative stress that activates signalling pathways in multiple forms and can therefore contribute to human diseases. Thus, the current study aims to evaluate the signalling pathway involved in cell death after testicular hyperthermia. For this purpose, 32 mice were equally divided into four groups; I: Control; II, III and IV, Scrotal hyperthermia in which the testes are exposed to water at 43°C for 20 min every other day, respectively, 15, 10 and 5 times. Then, animals were euthanized and testicular tissue samples were isolated to evaluate protein expression as well as germ cell gene marker expression by Western blot and real‐time PCR tests. Our data showed that the protein expression of Caspase‐1, Beclin1, Atg7, Mlkl and Acsl4 together with the expression of Caspase‐1, Beclin1, Atg7, Mlkl and Acsl4 genes was significantly up‐regulated in scrotal hyperthermia‐induced mice. In conclusion, the present study showed that heat stress disrupts spermatogenesis by activating several non‐apoptotic signalling pathways in testicular tissue.
Bibliography:Amirhosein Hasani and, Amirreza Khosravi contributed equally to this work.
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ISSN:0303-4569
1439-0272
DOI:10.1111/and.14320