Cell surface properties and biofilm formation of pathogenic bacteria

• Published in: Food science and biotechnology Vol. 24; no. 6; pp. 2257 - 2264
• Main Authors: Choi, Na-Young, Bae, Young-Min, Lee, Sun-Young
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.12.2015; 한국식품과학회
• Subjects: ammonium sulfate; Bacillus cereus; bacteria; bacterial adhesion; biofilm; carbohydrates; Chemistry; Chemistry and Materials Science; contact angle; correlation; DNA; Escherichia coli; Escherichia coli O157; Food Science; hydrocarbons; hydrophobic interaction chromatography; hydrophobicity; Listeria monocytogenes; Nutrition; pathogens; proteins; Pseudomonas aeruginosa; Staphylococcus aureus; 식품과학; cell hydrophobicity; foodborne pathogen; biofilm
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-015-0301-y

Cell surface properties of slime production, cell hydrophobicity (CH), colony spreading, auto-aggregation, and biofilm formation (BF) of pathogens were investigated. Staphylococcus aureus, Pseudomonas aeruginosa , and Listeria monocytogenes generally formed strong biofilms and were positive slime producers. The extracellular polymeric substances isolated from agar-grown biofilms of Bacillus cereus and P. aeruginosa contained a high proportion of carbohydrates, proteins, and extracellular DNA. High levels of CH were observed for all pathogens when hydrocarbons were combined with ammonium sulfate (AS). Levels of hydrophobicity based on bacterial adhesion to hydrocarbons using nonane with AS were positively correlated with hydrophobic interaction chromatography assay and contact-angle measurement results. BF and CH values of all pathogens, except E. coli O157:H7 and B. cereus , were highly correlated. Levels of pathogen hydrophobicity differed depending on the measurement method. Factors affecting BF were different depending upon the pathogen.