Methyl jasmonate-induced compositional changes of volatile organic compounds in Polygonum minus leaves

• Published in: Journal of plant physiology Vol. 240; p. 152994
• Main Authors: Rahnamaie-Tajadod, Reyhaneh, Goh, Hoe-Han, Mohd Noor, Normah
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.09.2019; Elsevier Science Ltd
• Subjects: Acetates - administration & dosage; Acetates - pharmacology; Aldehydes; Aquatic plants; Aromatic compounds; Biosynthesis; Chemical composition; Composition; Cyclopentanes - administration & dosage; Cyclopentanes - pharmacology; Elicitation; Gas chromatography; Herbal medicine; Leaves; Mass spectrometry; Mass spectroscopy; Medicinal plants; Metabolites; Metabolomics; Methyl jasmonate; Monoterpenes; Organic compounds; Oxylipins - administration & dosage; Oxylipins - pharmacology; Persicaria minor; Phenolic compounds; Phenols; Plant Growth Regulators - pharmacology; Plant Leaves - chemistry; Plant Leaves - drug effects; Polygonum; Polygonum - chemistry; Polygonum - drug effects; Secondary metabolites; Sesquiterpenes; Solid phase methods; Solid phases; SPME–GC–MS; Terpenes; VOCs; Volatile compounds; Volatile organic compounds; Volatile Organic Compounds - metabolism; Volatiles; ST; GC–MS; SPME–GC–MS; volatile organic compounds; MT; PLS-DA; HS; MeJA; VOC; Persicaria minor; Methyl jasmonate; PCA; GLV; SPME; JA; MVA; Elicitation; HD; VIP; Terpenes; RT-qPCR; PDMS
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2019.152994

Polygonum minus Huds. is a medicinal aromatic plant rich in terpenes, aldehydes, and phenolic compounds. Methyl jasmonate (MeJA) is a plant signaling molecule commonly applied to elicit stress responses to produce plant secondary metabolites. In this study, the effects of exogenous MeJA treatment on the composition of volatile organic compounds (VOCs) in P. minus leaves were investigated by using a metabolomic approach. Time-course changes in the leaf composition of VOCs on days 1, 3, and 5 after MeJA treatment were analyzed through solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC–MS). The VOCs found in MeJA-elicited leaves were similar to those found in mock-treated leaves but varied in quantity at different time points. We focused our analysis on the content and composition of monoterpenes, sesquiterpenes, and green leaf volatiles (GLVs) within the leaf samples. Our results suggest that MeJA enhances the activity of biosynthetic pathways for aldehydes and terpenes in P. minus. Hence, the production of aromatic compounds in this medicinal herb can be increased by MeJA elicitation. Furthermore, the relationship between MeJA elicitation and terpene biosynthesis in P. minus was shown through SPME–GC–MS analysis of VOCs combined with transcriptomic analysis of MeJA-elicited P. minus leaves from our previous study.