Can Storage Stability and Simulated Gastrointestinal Behavior Change the Cytotoxic Effects of Concentrated Guava Leaves Extract against Human Lung Cancer Cells?

• Published in: Membranes (Basel) Vol. 14; no. 5; p. 113
• Main Authors: Demaman Arend, Giordana, Verruck, Silvani, Zanchett Schneider, Naira Fernanda, Oliveira Simões, Cláudia Maria, Tres, Marcus Vinícius, Prudêncio, Elane Schwinden, Cunha Petrus, José Carlos, Rezzadori, Katia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.05.2024
• Subjects: Acids; antioxidant activity; Antioxidants; Care and treatment; Complications and side effects; cytoprotective effect; Cytotoxicity; Diagnosis; Digestion; Digestive system; Energy consumption; Functional foods & nutraceuticals; Gastrointestinal tract; Guava; Health aspects; Lung cancer; lung carcinoma; Medicine, Botanic; Medicine, Herbal; Nanofiltration; Nanotechnology; Patient outcomes; Phase transitions; Phenolic compounds; Phenols; Psidium guajava L. leaves; Refrigeration; Shelf life; Storage conditions; Storage stability; Temperature; Vero cells; Brazil; United States > US; cytoprotective effect; phenolic compounds; Psidium guajava L. leaves; lung carcinoma; antioxidant activity
• ISSN: 2077-0375; 2077-0375
• DOI: 10.3390/membranes14050113

The influence of storage stability and simulated gastrointestinal behavior of different extracts of guava leaves extracts (NC: not concentrated, and C10 and C20: concentrated by nanofiltration) was evaluated based on their total phenolic compound (TPC) contents and antioxidant activity as well as on their cytotoxic effects on A549 and Vero cells. The results showed that C10 and C20 presented high stability for 125 days probably due to their high TPC contents and antioxidant activity. The simulated gastrointestinal behavior modified their TPC contents; however, after all digestion steps, the TPC values were higher than 70%, which means that they were still available to exert their bioactivities. Additionally, the cytotoxic effects of these extracts were evaluated before and after the simulated gastrointestinal behavior or under different storage conditions. C10 presented the best selectivity indices (SI) values (IC50 Vero cells/IC50 A549 cells) at both conditions suggesting that it can be considered a potential extract to be developed as a functional food due to its resistance to the gastrointestinal digestion and storage conditions tested.