Assessing RNA quality in postmortem human brain tissue

• Published in: Experimental and molecular pathology Vol. 84; no. 1; pp. 71 - 77
• Main Authors: Chevyreva, Ia, Faull, Richard L.M., Green, Colin R., Nicholson, Louise F.B.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.02.2008; Elsevier
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Agonal state; Biological and medical sciences; Brain - anatomy & histology; Brain - pathology; Brain Chemistry; Cerebellar; Female; Humans; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Microarray; Microarray Analysis; Middle Aged; Neurodegenerative Diseases - genetics; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Postmortem Changes; RNA - analysis; RNA quality; RNA Stability; Time Factors; Tissue pH; Cerebellar; Tissue pH; RNA quality; Microarray; Agonal state; Cerebellum; Human; RNA; Central nervous system; Postmortem; Encephalon; Tissue; Anatomic pathology; Quality; pH
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2007.08.019

The development of microarrays for the screening of gene expression has highlighted the importance of obtaining high quality RNA. We have investigated whether it was possible to obtain RNA of sufficiently good quality from postmortem human tissue using samples obtained from the New Zealand Neurological Foundation Human Brain Bank. We have investigated the effect of PM delay, the duration of the agonal state, the tissue pH, the age at death and the sex of the patient on the quality of normal human brain tissue and tissue from patients with various neurodegenerative conditions. Postmortem delay was shown to affect the RNA quality adversely, but the magnitude of the effect was small. While cerebellar RNA quality was not always an exact predictor of the RNA quality in other brain regions of interest, it was shown to have some predictive value and can be used as a preliminary indicator. The principle finding was that RNA quality is most strongly affected by the pH of the tissue, with both the pH and the RNA quality being influenced by the mode of death.