Lutaonella thermophila gen. nov., sp. nov., a moderately thermophilic member of the family Flavobacteriaceae isolated from a coastal hot spring

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 8; pp. 2069 - 2073
• Main Authors: Arun, A. B, Chen, Wen-Ming, Lai, Wei-An, Chou, Jui-Hsing, Shen, Fo-Ting, Rekha, P. D, Young, Chiu-Chung
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.2009; Society for General Microbiology
• Subjects: Aerobiosis; Amino Acids - metabolism; Bacterial Typing Techniques; Bacteriology; Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Bacteroidetes - physiology; Base Composition; Biological and medical sciences; Carbohydrate Metabolism; Carboxylic Acids - metabolism; Cellulophaga; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Flavobacteriaceae; Fundamental and applied biological sciences. Psychology; Hot Springs - microbiology; Locomotion; Microbiology; Molecular Sequence Data; Phylogeny; Pigments, Biological - biosynthesis; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Taiwan; Taiwan; ISEW, Taiwan; New genus; Flavobacteriaceae; Taxonomy; Bacteria; Thermophily; New species; Hot source; Coastal zone
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.005256-0

1 Yenepoya Research Center, Yenepoya University, University Rd, Deralakatee, Mangalore, Karnataka state, India 2 Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC 3 College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC Correspondence Chiu-Chung Young ccyoung{at}mail.nchu.edu.tw A yellow-pigmented, Gram-staining-negative, aerobic, non-motile, moderately thermophilic, rod-shaped bacterium, CC-MHSW-2 T , was isolated from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. The 16S rRNA gene sequence analysis demonstrated that it shared <93.2 % sequence similarity with Aquimarina species. The organism was unable to produce acid from carbohydrates, but it could utilize a number of organic acids and amino acids. Menaquinone 6 (MK-6) was the major respiratory quinone and iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 (comprising C 16 : 1 7 c and/or C 16 : 1 6 c ) were the predominant fatty acids. This fatty acid profile distinguished the isolate from members of the genera Aquimarina , Tamlana , Zhouia , Leeuwenhoekiella and Cellulophaga . The DNA G+C content of strain CC-MHSW-2 T was 39.7±1 mol%. On the basis of the 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain CC-MHSW-2 T represents a novel genus and species in the family Flavobacteriaceae , for which the name Lutaonella thermophila gen. nov., sp. nov. is proposed. The type strain is CC-MHSW-2 T (=KCTC 22538 T =JCM 15069 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CC-MHSW-2 T is EU287913 .