Anti-aging effects of Ribes meyeri anthocyanins on neural stem cells and aging mice
Aging is associated with neurological impairment and cognitive decline. Flavonoids are very promising in anti-aging research in mouse models. anthocyanins are rich in abundant flavonoids, but their anti-aging biological activities remain unknown. In this study, we prepared an anthocyanin extract and...
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Published in | Aging (Albany, NY.) Vol. 12; no. 17; pp. 17738 - 17753 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Impact Journals
12.09.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Aging is associated with neurological impairment and cognitive decline. Flavonoids are very promising in anti-aging research in mouse models.
anthocyanins are rich in abundant flavonoids, but their anti-aging biological activities remain unknown. In this study, we prepared an
anthocyanin extract and analyzed its effects on neural stem cell (NSC) senescence
and
. We isolated mouse NSCs and used cell counting kit-8 (CCK-8), cell cycle, reactive oxygen species (ROS), and immunofluorescence methods to analyze the anti-aging effects of
anthocyanins as well as naringenin (Nar), which metabolic analysis revealed as an important flavonoid in
anthocyanins. RNA-sequencing (RNA-seq) and enzyme-linked immuno sorbent assay (ELISA) methods were also used to investigate Nar-specific mechanisms of anti-aging. After
anthocyanin treatment, NSC proliferation accelerated, and NSCs had decreased senescence markers, and reduced P16
expression.
anthocyanin treatment also reversed age-dependent neuronal loss
and
. Nar blocked mNSC aging
and improved spatial memory and cognitive abilities in aging mice through downregulation of plasma TNF-α protein. These findings suggest that
anthocyanins increase NSC proliferation and improve neurogenesis with aging via Nar-induced reductions in TNF-α protein levels
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Equal contribution |
ISSN: | 1945-4589 1945-4589 |
DOI: | 10.18632/AGING.103955 |