First detection of TEM-116 extended-spectrum β-lactamase in a Providencia stuartii isolate from a Tunisian hospital

• Published in: Indian journal of medical microbiology Vol. 29; no. 3; pp. 258 - 261
• Main Authors: Lahlaoui, H, Dahmen, S, Moussa, MB, Omrane, B
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.07.2011
• Subjects: Anti-Bacterial Agents - pharmacology; beta-Lactam Resistance; beta-Lactamases - genetics; Cephalosporins - pharmacology; Conjugation, Genetic; Enterobacteriaceae Infections - microbiology; Escherichia coli - genetics; Extended spectrum β-lactamases; Gene Transfer, Horizontal; Hospitals; Humans; Plasmids; Polymerase Chain Reaction; Providencia - drug effects; Providencia - enzymology; Providencia - genetics; Providencia - isolation & purification; Providencia stuartii; Sequence Analysis, DNA; TEM-116; Tunisia; Tunisia; Extended spectrum β-lactamases; TEM-116; tunisia; Providencia stuartii
• ISSN: 0255-0857; 1998-3646
• DOI: 10.4103/0255-0857.83909

Purpose: To study the resistance to third-generation cephalosporins in Providencia stuartii strain isolated from hospitalized patient in Tunisia and to identify the responsible genes Materials and Methods: This strain was analysed by PCR and sequencing to identify the genes responsible for the β-lactamase resistance phenotypes. The transferability of the phenotypes was tested by conjugation to Escherichia coli J53. The isoelectric point was determinate by isoelectrofocalisation. Results: This resistance was carried by a 60 kb plasmid that encoded a β-lactamase with a pI of 5.4. This β-lactamase revealed identity with the blaTEM-1 gene encoding the TEM-1 β-lactamase, except for a replacement of the Val residue at position 84 by Ile, and the Ala residue at position 184 by Val. These two mutations were encountered in TEM-116 β-lactamase. Conclusion: This study demonstrates the first description of TEM-116 in the P. stuartii species in the world and the first one in a Tunisian hospital.