The development of a latex agglutination inhibition assay for the determination of foetal calf serum in viral vaccines

A simple but specific, sensitive and reproducible latex agglutination inhibition assay for the determination of foetal calf sera in viral vaccines has been developed and standardized. The detection limit was at nanogram level. The assay procedure requires two pipetting steps, a short centrifugation...

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Bibliographic Details
Published inBiologicals Vol. 18; no. 1; pp. 45 - 48
Main Authors Sladoljev, S., Treščec, Andja, Cvetković, Maja, Beck, M., Juzbašić, Mirjam
Format Journal Article
LanguageEnglish
Published Kent Elsevier Ltd 1990
Elsevier Science
Subjects
Animals
Biological and medical sciences
Blood
Cattle
Drug Contamination - prevention & control
Fundamental and applied biological sciences. Psychology
Horses
Humans
Latex Fixation Tests - methods
Microbiology
Rabbits
Reproducibility of Results
Sensitivity and Specificity
Species Specificity
Techniques used in virology
Viral Vaccines - analysis
Virology
Performance evaluation
Bovine
Biosynthesis
Vaccine
Virus
Vertebrata
Specificity
Mammalia
Fetus
Serum
Artiodactyla
Detection
Ungulata
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Summary:A simple but specific, sensitive and reproducible latex agglutination inhibition assay for the determination of foetal calf sera in viral vaccines has been developed and standardized. The detection limit was at nanogram level. The assay procedure requires two pipetting steps, a short centrifugation stage and the use of a spectrophotometer.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1045-1056
1095-8320
DOI:10.1016/1045-1056(90)90069-C