Effects of process parameters on EPA and DHA concentrate production from Atlantic salmon by-product oil: Optimization and characterization

Supercritical carbon dioxide (SC-CO 2 ) extracted Atlantic salmon frame bone oil (SFBO) was used for Eicosapentaenoic acid and Docosahexaenoic acid (EPA-DHA) concentrate production by urea complexation. Urea/fatty acids (2.5 to 4.0 w/w), crystallization temperature (−24 to −8 °C) and crystallization...

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Published inThe Korean journal of chemical engineering Vol. 34; no. 8; pp. 2255 - 2264
Main Authors Haq, Monjurul, Getachew, Adane Tilahun, Saravana, Periaswamy Sivagnanam, Cho, Yeon-Jin, Park, Seul-Ki, Kim, Min-Jung, Chun, Byung-Soo
Format Journal Article
LanguageEnglish
Published New York Springer US 01.08.2017
Springer Nature B.V
한국화학공학회
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Summary:Supercritical carbon dioxide (SC-CO 2 ) extracted Atlantic salmon frame bone oil (SFBO) was used for Eicosapentaenoic acid and Docosahexaenoic acid (EPA-DHA) concentrate production by urea complexation. Urea/fatty acids (2.5 to 4.0 w/w), crystallization temperature (−24 to −8 °C) and crystallization time (8 to 24 h) were studied by Box-Behnken Design (BBD) to maximize EPA-DHA content. Highest EPA-DHA content was 60.63% at urea/fatty acids 4.0 w/w, crystallization temperature −15.67 °C and crystallization time 8 h. EPA-DHA concentrate showed improvement of EPA-DHA from 6.39% in SFBO to 62.34%, increase of astaxanthin content from 21.33 μg/g in SFBO to 44.69 μg/g in EPA-DHA concentrate, no residual urea and reduction of many off-flavor compounds. The EPA-DHA yield showed an inverse relation with the urea/fatty acids, whereas its concentration increased proportionally with urea/fatty acids. Therefore, EPA-DHA concentrate produced from SFBO by urea complexation may be an efficient technique to provide ω -3 polyunsaturated fatty acids to the consumers.
ISSN:0256-1115
1975-7220
DOI:10.1007/s11814-016-0362-5