Effects of process parameters on EPA and DHA concentrate production from Atlantic salmon by-product oil: Optimization and characterization

Supercritical carbon dioxide (SC-CO 2 ) extracted Atlantic salmon frame bone oil (SFBO) was used for Eicosapentaenoic acid and Docosahexaenoic acid (EPA-DHA) concentrate production by urea complexation. Urea/fatty acids (2.5 to 4.0 w/w), crystallization temperature (−24 to −8 °C) and crystallization...

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Published inThe Korean journal of chemical engineering Vol. 34; no. 8; pp. 2255 - 2264
Main Authors Haq, Monjurul, Getachew, Adane Tilahun, Saravana, Periaswamy Sivagnanam, Cho, Yeon-Jin, Park, Seul-Ki, Kim, Min-Jung, Chun, Byung-Soo
Format Journal Article
LanguageEnglish
Published New York Springer US 01.08.2017
Springer Nature B.V
한국화학공학회
Subjects
Astaxanthin
Biotechnology
Carbon dioxide
Catalysis
Chemistry
Chemistry and Materials Science
Complexation
Consumers
Crystallization
Docosahexaenoic acid
Eicosapentaenoic acid
Fatty acids
Industrial Chemistry/Chemical Engineering
Materials Science
Process parameters
Salmon
Separation Technology
Thermodynamics
화학공학
Volatile Organic Compounds
Docosahexaenoic Acid
Optimization
Salmon Frame Bone Oil
Eicosapentaenoic Acid
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Summary:Supercritical carbon dioxide (SC-CO 2 ) extracted Atlantic salmon frame bone oil (SFBO) was used for Eicosapentaenoic acid and Docosahexaenoic acid (EPA-DHA) concentrate production by urea complexation. Urea/fatty acids (2.5 to 4.0 w/w), crystallization temperature (−24 to −8 °C) and crystallization time (8 to 24 h) were studied by Box-Behnken Design (BBD) to maximize EPA-DHA content. Highest EPA-DHA content was 60.63% at urea/fatty acids 4.0 w/w, crystallization temperature −15.67 °C and crystallization time 8 h. EPA-DHA concentrate showed improvement of EPA-DHA from 6.39% in SFBO to 62.34%, increase of astaxanthin content from 21.33 μg/g in SFBO to 44.69 μg/g in EPA-DHA concentrate, no residual urea and reduction of many off-flavor compounds. The EPA-DHA yield showed an inverse relation with the urea/fatty acids, whereas its concentration increased proportionally with urea/fatty acids. Therefore, EPA-DHA concentrate produced from SFBO by urea complexation may be an efficient technique to provide ω -3 polyunsaturated fatty acids to the consumers.
ISSN:0256-1115
1975-7220
DOI:10.1007/s11814-016-0362-5