Covalently linked peptides for enzyme-linked immunosorbent assay

A general method is described, by which synthetic peptides are covalently linked via their carboxyl group to microtiter plates (CovaLink) for enzyme-linked immunosorbent assay (ELISA). Plates were prepared by this method with an angiotensin II peptide and with an HIV-2 peptide and attachment detecte...

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Bibliographic Details
Published inJournal of immunological methods Vol. 131; no. 1; p. 99
Main Authors Søndergård-Andersen, J, Lauritzen, E, Lind, K, Holm, A
Format Journal Article
LanguageEnglish
Published Netherlands 20.07.1990
Subjects
Angiotensin II - analysis
Carboxylic Acids
Dose-Response Relationship, Immunologic
Enzyme-Linked Immunosorbent Assay - methods
HIV Antigens - analysis
HIV Infections - immunology
HIV-2 - immunology
Humans
Peptides - analysis
Peptides - immunology
Plastics
Retroviridae Proteins - analysis
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Summary:A general method is described, by which synthetic peptides are covalently linked via their carboxyl group to microtiter plates (CovaLink) for enzyme-linked immunosorbent assay (ELISA). Plates were prepared by this method with an angiotensin II peptide and with an HIV-2 peptide and attachment detected by rabbit anti-angiotensin serum and with a positive serum from an HIV-2-infected patient, respectively, using the common ELISA procedure in the last steps. The method is simple to perform, it constitutes an alternative to the common ELISA method, and eliminates the risk of inadvertent loss of peptide during the procedure. The method is highly reproducible and has a high sensitivity. It may be used for either antigen or antibody detection.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(90)90238-Q