Temporal changes in intracellular distribution of protein kinase C in Swiss 3T3 cells during mitogenic stimulation with insulin-like growth factor I and bombesin: Translocation to the nucleus follows rapid changes in nuclear polyphosphoinositides

• Published in: Biochemical and biophysical research communications Vol. 177; no. 1; pp. 480 - 487
• Main Authors: Martelli, A.M., Neri, L.M., Gilmour, R.S., Barker, R.J., Huskisson, N.S., Manzoli, F.A., Cocco, L.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 31.05.1991; Elsevier
• Subjects: Amino Acid Sequence; Animals; Biological and medical sciences; Bombesin - pharmacology; Cell Compartmentation - drug effects; Cell Division - drug effects; Cell Line; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cell physiology; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Immunoblotting; Immunohistochemistry; Insulin-Like Growth Factor I - pharmacology; Kinetics; Mice; Molecular and cellular biology; Molecular Sequence Data; Molecular Weight; Nuclear Proteins - isolation & purification; Nuclear Proteins - metabolism; Phosphatidylinositol Phosphates; Phosphatidylinositols - metabolism; Protein Kinase C - metabolism; Responses to growth factors, tumor promotors, other factors; Time Factors; PBS; IGF-I; PS; PKC; InsP3; PtdInsP; DAG; EDTA; SDS-PAGE; BSA; PMSF; PDGF; NGS; PtdInsP2; Cell culture; Intracellular transport; Protein kinase C; Bombesin; Somatomedin C; Immunocytochemistry; Rodentia; Peptide hormone; 3T3-Cell line; Stimulation; Neuropeptide; Biological activity; Vertebrata; Mammalia; Mouse; Polypeptide; Immunoblotting assay; Growth factor
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(91)92009-9

Using a polyclonal antibody raised against a synthetic peptide of the catalytic region of protein kinase C, we have carried out a combined immunocytochemical and immunochemical analysis to follow the subcellular localisation of this enzyme in response to mitogenic stimulation with insulin-like growth factor I and bombesin. These investigations show a time dependent translocation of protein kinase C from the cytoplasm to the nucleus since 5 min stimulation reaching a maximal effect after 45 min. These results show clearly that mitogen induced translocation of protein kinase C to the nucleus follows temporally the earlier changes in nuclear polyphosphoinositide metabolism previously demonstrated.