Preparation of photoreactive azidophenyl hyaluronic acid derivative: Protein immobilization for medical applications

• Published in: Macromolecular research Vol. 21; no. 2; pp. 216 - 220
• Main Authors: Lee, Hyung-Jae, Park, Shin-Hye, Seo, Si-Yoong, Cho, Young-Min, Woo, Hee Dong, Ito, Yoshihiro, Son, Tae-Il
• Format: Journal Article
• Language: English
• Published: Heidelberg The Polymer Society of Korea 01.02.2013; 한국고분자학회
• Subjects: Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Complex Fluids and Microfluidics; Nanochemistry; Nanotechnology; Physical Chemistry; Polymer Sciences; Soft and Granular Matter; 고분자공학; protein immobilization; 4-azidoaniline; photoreactive; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; hyaluronic acid
• ISSN: 1598-5032; 2092-7673
• DOI: 10.1007/s13233-013-1016-7

Photoreactive azidophenyl hyaluronic acid (Az-HA) derivative was synthesized to immobilize proteins such as epidermal growth factor (EGF) and bone morphogenetic protein (BMP). The photosensitizing group, 4-azidoaniline, was introduced to the carboxyl group of hyaluronic acid by a water soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). To characterize the synthesized Az-HA, Fourier transform infrared (FTIR), proton nuclear magnetic resonance ( 1 H NMR) and ultraviolet/visible (UV/vis) absorbance spectroscopy were performed. We demonstrated protein immobilization by using fluorescein isothiocyanate conjugated albumin (FITCBSA) on Az-HA derivative surfaces that were patterned with micro scale. Cytotoxicity and relative cell proliferation tests of the Az-HA derivative were performed on an MG-63 human osteoblast cell line for biocompatibility.