Detection of Enteroviral RNA Sequences in Different Water Samples

Viruses were isolated from different water samples by Sterivex-Filtration. The nucleic acids were isolated in the filter unit and purified by phenol-chloroform extraction and ethanol precipitation. Reverse transcription and polymerase chain reaction (RT/PCR) were performed with primer pairs compleme...

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Bibliographic Details
Published inWater Science & Technology Vol. 27; no. 3-4; pp. 219 - 222
Main Authors Tougianidou, D, Botzenhart, K
Format Journal Article Conference Proceeding
LanguageEnglish
Published London IWA Publishing 01.01.1993
Subjects
Acids
Chloroform
Ethanol
Gene sequencing
Mineral oils
Nucleic acids
Phenols
Polymerase chain reaction
Ribonucleic acid
RNA
Viruses
Water analysis
Water purification
Water sampling
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Summary:Viruses were isolated from different water samples by Sterivex-Filtration. The nucleic acids were isolated in the filter unit and purified by phenol-chloroform extraction and ethanol precipitation. Reverse transcription and polymerase chain reaction (RT/PCR) were performed with primer pairs complementary to sequences of the enteroviral 5’ noncoding region. Amplified sequences were detected by hybridisation with an oligonucleotide complementary to a part of the PCR product. The test system seems to be sensitive and specific In the detection of enteroviral RNA.
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ISBN:0080422071
9780080422077
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.1993.0349