Glutamate dehydrogenase: Some properties of the rat brain enzyme from different cellular compartments

• Published in: Comparative biochemistry and physiology. C, Comparative pharmacology Vol. 97; no. 2; pp. 265 - 267
• Main Authors: Arce, C., Canadas, S., Oset-Gasque, M.J., Castro, E., González, M.P.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 1990; Pergamon
• Subjects: Adenosine Diphosphate - pharmacology; Animals; Biochemistry and metabolism; Biological and medical sciences; Brain - cytology; Brain - enzymology; Central nervous system; Enzyme Activation - drug effects; Fundamental and applied biological sciences. Psychology; Glutamate Dehydrogenase - metabolism; Glutamates - metabolism; Glutamic Acid; Neuroglia - enzymology; Neurons - enzymology; Rats; Rats, Inbred Strains; Synaptosomes - enzymology; Vertebrates: nervous system and sense organs; Glutamate dehydrogenase; Brain; Glial cell; Rat; Enzyme; Rodentia; Central nervous system; Biosynthesis; Degradation; Vertebrata; Regulation(control); Synaptosome; Mammalia; Enzymatic activity
• ISSN: 0306-4492; 0742-8413
• DOI: 10.1016/0742-8413(90)90139-Z

1. Differences in the GDH activity of neuronal, glial cells and synaptosomes were detected. 2. The enzyme was measured in both directions: synthesis and degradation of glutamate. 3. Synaptosomes were the region with the highest GDH activity. 4. ADP plays an imporatant role in the regulation of the reaction sense. 5. This effector produced higher activation on the enzyme measured in the direction of glutamate synthesis than in the sense of its degradation. 6. The enhancement produced by ADP was dependent on the enzyme localization. The ADP effect is discussed.