Differential staining of normal and neoplastic mouse lung epithelia by succinate dehydrogenase histochemistry

• Published in: Cancer letters Vol. 52; no. 3; pp. 219 - 227
• Main Authors: Thaete, L.G., Malkinson, A.M.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 31.07.1990; Elsevier
• Subjects: adenocarcinoma; Adenocarcinoma - enzymology; Adenocarcinoma - pathology; Adenoma - enzymology; Adenoma - pathology; Ageing, cell death; Aging; alveolar type II pneumocytes; Animals; Biological and medical sciences; Biomarkers - analysis; bronchiolar clara cells; Carcinoma - enzymology; Carcinoma - pathology; Cell physiology; Epithelial Cells; Epithelium - enzymology; Epithelium - pathology; Female; Fundamental and applied biological sciences. Psychology; Histocytochemistry; Lung - cytology; Lung - enzymology; Lung - growth & development; Lung - pathology; Lung Neoplasms - enzymology; Lung Neoplasms - pathology; Male; Mice; Mice, Inbred A; Molecular and cellular biology; mouse; pulmonary adenomas; Succinate Dehydrogenase - analysis; Succinate Dehydrogenase - metabolism; succinate dehydrogenase histochemistry; mouse; adenocarcinoma; succinate dehydrogenase histochemistry; pulmonary adenomas; bronchiolar clara cells; alveolar type II pneumocytes; Adenocarcinoma; Alveolar type II cell; Vertebrata; Succinate dehydrogenase; Mammalia; Enzymatic activity; Mouse; Enzyme; Lung; Rodentia; Tumor; Histochemistry
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/0304-3835(90)90190-9

The cell type of origin of primary mouse lung tumors was investigated histochemically. Succinate dehydrogenase (SDH) histochemistry readily differentiated bronchiolar epithelial (Clara and ciliated) cells from alveolar type II pneumocytes in mice aged 10 days through adult. Correspondingly, freshly-isolated Clara cells stained intensely but type II cells did not. Urethane-induced papillary adenomas exhibited intense SDH staining while alveolar adenomas stained very lightly. The relative SDH activities of these two types of pulmonary adenomas is consistent with the hypothesis that alveolar tumors arise from type II pneumocytes and papillary tumors from Clara cells.