Improved enzyme-linked immunosorbent assay using surface-adhesive antibody-oriented immobilizing biolinker: a proof-of-concept study

• Published in: Biotechnology and bioprocess engineering Vol. 29; no. 3; pp. 543 - 550
• Main Authors: Lee, Ae Sol, Heo, Hye Ryoung, Kim, Chang Sup, Cha, Hyung Joon
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.06.2024; Springer Nature B.V; 한국생물공학회
• Subjects: Adhesives; Adsorption; Antibodies; Antigens; Biotechnology; Chemistry; Chemistry and Materials Science; Engineering; Enzyme-linked immunosorbent assay; Enzymes; Immobilization; Immunoassays; Industrial and Production Engineering; Laboratories; mussels; Protein A; Proteins; Reproducibility; Research Paper; Sensitivity; 생물공학; Enzyme-linked immunosorbent assays; Surface adhesive antibody-oriented immobilizing linker; Sensitivity; Reproducibility; Orientated antibody immobilization
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-024-00093-7

Enzyme-linked immunosorbent assays (ELISA) have been widely used to detect disease-related antigens in clinical and research laboratories. One of the main drawbacks of ELISA is the utilization of physical adsorption for immobilizing antibodies on a surface, causing low sensitivity, reproducibility, and precision. In this study, we applied a BC-MAP linker composed of antibody-immobilizing BC domains of protein A and surface-adhesive mussel adhesive protein (MAP) to an ELISA platform to overcome these limitations. The performance of ELISA using BC-MAP linker was compared with that of untreated ELISA. BC-MAP proteins were reproducibly coated to the surface while exposing BC domains, resulting in twofold higher sensitivity and improved reproducibility of ELISA compared to the untreated ELISA utilizing physical adsorption of antibodies. Thus, the proposed method could be successfully used in ELISA platforms to diagnose and manage diseases.