Rapid Determination of α-Hederin and Hederacoside C in Extracts of Hedera helix Leaves Available in the Czech Republic and Poland

• Published in: Natural product communications Vol. 10; no. 9; pp. 1529 - 1531
• Main Authors: Havlíková, Lucie, Macáková, Kateřina, Opletal, Lubomír, Solich, Petr
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.09.2015
• Subjects: Chromatography, High Pressure Liquid - methods; Czech Republic; Hedera - chemistry; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - chemistry; Plant Extracts - chemistry; Plant Leaves - chemistry; Poland; Reproducibility of Results; Saponins - chemistry; Poland; Czech Republic; α-Hederin; Hedera helix leaves; Common ivy; Quantitative estimation; Hederoside C; HPLC
• ISSN: 1934-578X; 1555-9475
• DOI: 10.1177/1934578X1501000910

Leaf extracts of Hedera helix L. are widely used in the treatment of upper respiratory diseases. The saponins α-hederin and hederacoside C are considered to be the main compounds responsible for the biological activity. α-Hederin and hederacoside C were determined in H. helix leaf extracts using a fast, simple and validated HPLC method. An XTerra MS C18 column and mobile phase composed of 10 mM ammonium acetate at pH 8.5 (adjusted with triethylamine) and acetonitrile were used for the chromatography at 1.2 mL min−1. The column was kept at 30°C. Detection was performed at 220 nm. An approach utilizing a basic pH of the aqueous part of the mobile phase enabled analysis in 5 minutes in isocratic mode. The method was validated and used for the quality control of H. helix leaf ethanolic extracts.