Association of TNF-α, IL-10 and IL-6 promoter polymorphisms in pulmonary tuberculosis patients and their household contacts of younger age group

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 56; pp. 20 - 26
• Main Authors: Joshi, Lavanya, Chelluri, Lakshmi Kiran, Valluri, Vijayalakshmi, Gaddam, Sumanlatha
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2018; Elsevier Science Ltd
• Subjects: Adolescent; Adult; Age Factors; Biomarkers; Case-Control Studies; Clinical outcomes; Family Characteristics; Genetic Markers - genetics; Genetic Predisposition to Disease - genetics; Genotypes; Genotyping Techniques; Health risks; Household contacts; Households; Humans; IL-10; IL-6; Interleukin 10; Interleukin 6; Interleukin-10 - genetics; Interleukin-6 - genetics; Latent Tuberculosis - genetics; MDR; Patients; Polymorphism, Single Nucleotide - genetics; Promoter Regions, Genetic - genetics; Risk assessment; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Studies; TNF-α; Tuberculosis; Tuberculosis, Pulmonary - genetics; Tuberculosis, Pulmonary - immunology; Tumor Necrosis Factor-alpha - genetics; Tumor necrosis factor-α; Young Adult; MDR; Single nucleotide polymorphisms; TNF-α; Household contacts; IL-10; IL-6
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2017.12.001

•Single nucleotide polymorphisms (SNPs) of cytokine genes are one of the major factors in determining the susceptibility of the disease.•The study of SNPs may be useful for identification of genotypes as biomarkers.•The gene interactions of different cytokine genes at different positions may help us to identify the high risk genotypes.•The high risk genotypes of IL10 and IL6 may be used for identification of latency in household contacts. Single nucleotide polymorphisms (SNPs) of cytokine genes have been found to be involved in the clinical outcome of Tuberculosis. The present study was aimed to identify the high risk genotypes in Tuberculosis patients and their household contacts. A total of 490 subjects were studied which includes 150 active pulmonary tuberculosis patients (APTB), 190 household contacts (HHC) and 150 healthy controls (HC). The SNPs of TNF-α (-308A/G), IL-10(-1082G/A) and IL-6(-174G/C) were performed by ARMs PCR. The IL-10 GA genotype showed significant association in APTB and HHC and was 2.3 times higher risk in APTB and 3.7 times in HHC compared to HCs. The A allele was found to be significantly associated with the risk of disease. The CC genotype of IL-6 was found to be significantly associated in APTB and an insignificant positive association in HHCs. The multifactor dimensionality reduction (MDR) analysis indicated that the genotypes of IL-6 were showing high risk with GA genotype of IL-10. In conclusion the gene interaction may be useful for identification of genotypes as biomarkers to distinguish high risk individuals.