Kinetic characterization of atrial natriuretic factor-sensitive particulate guanylate cyclase

• Published in: European journal of pharmacology Vol. 189; no. 4-5; p. 317
• Main Authors: Ivanova, K, Heim, J M, Gerzer, R
• Format: Journal Article
• Language: English
• Published: Netherlands 30.10.1990
• Subjects: Adrenal Cortex - drug effects; Adrenal Cortex - enzymology; Adrenal Cortex - metabolism; Amiloride - pharmacology; Animals; Atrial Natriuretic Factor - pharmacology; Cattle; Guanylate Cyclase - antagonists & inhibitors; Guanylate Cyclase - metabolism; In Vitro Techniques; Kinetics; Magnesium - pharmacology; Manganese - pharmacology; Membranes - drug effects; Membranes - metabolism; Octoxynol; Polyethylene Glycols - pharmacology; Rats

The present investigation describes kinetic characteristics of membrane-bound and Triton X-100-solubilized atrial natriuretic factor (ANF)-sensitive guanylate cyclase from bovine adrenal cortex. The kinetic analysis of both enzyme forms suggests that in the presence of manganese, ANF induces or stab...