Kinetic characterization of atrial natriuretic factor-sensitive particulate guanylate cyclase

• Published in: European journal of pharmacology Vol. 189; no. 4-5; p. 317
• Main Authors: Ivanova, K, Heim, J M, Gerzer, R
• Format: Journal Article
• Language: English
• Published: Netherlands 30.10.1990
• Subjects: Adrenal Cortex - drug effects; Adrenal Cortex - enzymology; Adrenal Cortex - metabolism; Amiloride - pharmacology; Animals; Atrial Natriuretic Factor - pharmacology; Cattle; Guanylate Cyclase - antagonists & inhibitors; Guanylate Cyclase - metabolism; In Vitro Techniques; Kinetics; Magnesium - pharmacology; Manganese - pharmacology; Membranes - drug effects; Membranes - metabolism; Octoxynol; Polyethylene Glycols - pharmacology; Rats
• ISSN: 0014-2999
• DOI: 10.1016/0922-4106(90)90125-H

The present investigation describes kinetic characteristics of membrane-bound and Triton X-100-solubilized atrial natriuretic factor (ANF)-sensitive guanylate cyclase from bovine adrenal cortex. The kinetic analysis of both enzyme forms suggests that in the presence of manganese, ANF induces or stabilizes at least two apparent GTP*Mn2(+)- and in addition two Mn2(+)-binding sites. Addition of the natriuretic drug amiloride favors this state. ATP increases the vmax in the presence of ANF for GTP*Mg2+, but not for GTP*Mn2+ as a substrate. With GTP*Mg2+, amiloride has no effect on basal or ANF-stimulated activity, but slightly reduces the effect of ATP. Under all conditions tested, the enzyme follows regular Michaelis-Menten kinetics in the presence of Mg2+ and exhibits positive cooperativity with Mn2+. Positive cooperativity is also retained after Triton extraction. The results indicate that Triton extraction has no major influence on the kinetic properties of particulate guanylate cyclase when the extraction procedure is done carefully. The data also support the suggestion that multiple interactions of subunits might occur upon activation of the enzyme by ANF in the presence of Mn2+.