Identification of a 200-kDa glycoprotein antigen of Saccharomyces cerevisiae

• Published in: Immunology letters Vol. 28; no. 3; pp. 181 - 185
• Main Authors: Heelan, Bridget T., Allan, Susan, Barnes, R.M.R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.1991; Elsevier
• Subjects: Antibodies; Antibodies, Fungal - immunology; Antigens, Fungal - isolation & purification; Biological and medical sciences; Crohn Disease - immunology; Crohn's disease; Cross Reactions - immunology; Electrophoresis, Polyacrylamide Gel; Gastroenterology. Liver. Pancreas. Abdomen; Glycoproteins; Glycoproteins - isolation & purification; Humans; Immunoblotting; Immunoglobulin A - immunology; Immunoglobulin G - immunology; Medical sciences; Molecular Weight; Other diseases. Semiology; Saccharomyces cerevisiae; Saccharomyces cerevisiae - immunology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Antibodies; Immunoblotting; Crohn's disease; Glycoproteins; Saccharomyces cerevisiae; Human; IgA; Antibody; Gel electrophoresis; Gut; IgG; Inflammatory disease; Fungi; Antigen; Crohn disease; Immunoblotting assay; Digestive diseases; Ascomycetes; Detection; ELISA assay; Thallophyta
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/0165-2478(91)90001-Q

SDS-PAGE analysis of the soluble proteins of the yeast Saccharomyces cerevisiae revealed a single predominant moiety of apparent size 200 kDa which was strongly periodic acid Schiff-positive. In immunoblotting experiments, human sera containing anti- S. cerevisiae antibodies were reactive only with this glycoprotein (gp200); this was demonstrated using sera containing IgG and IgA isotype-specific anti- S. cerevisiae antibodies. The antigenicity of gp200 was substantially reduced by periodate treatment. These results show that gp200 is a major glycoprotein antigen of S. cerevisiae which is immunogenic in man.