Interventions for control of Salmonella: Clearance of microbial growth from rubber picker fingers

• Published in: Poultry science Vol. 88; no. 6; pp. 1292 - 1298
• Main Authors: Arnold, J.W, Yates, I.E
• Format: Journal Article
• Language: English
• Published: Oxford, UK Poultry Science Association 01.06.2009; Oxford University Press
• Subjects: Animals; antimicrobial properties; bacterial adhesion; bacterial contamination; Biofilms - growth & development; broiler chickens; Chickens; Colony Count, Microbial; disease prevention; Disinfectants; disinfection; Escherichia coli; food contact surfaces; food contamination; Food Handling - methods; food microbiology; food processing equipment; food safety; Food-Processing Industry - instrumentation; Listeria monocytogenes; Meat - microbiology; meat carcasses; meat packing plants; microbial growth; Microscopy, Electron, Scanning; Pseudomonas aeruginosa; Rubber; rubber picker fingers; Salmonella; Salmonella - growth & development; Salmonella - ultrastructure; Salmonella enteritidis; Salmonella Food Poisoning - prevention & control; Staphylococcus aureus; bacterial contamination; disinfectant; broiler; rubber picker finger
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.2008-00391

A study was conducted to determine if a surface material with antimicrobial properties combined with an effective disinfectant could achieve total clearance of bacterial contamination. Before beginning the project, new rubber picker fingers collected from 3 processing facilities were tested for endogenous microflora. Five species of bacteria common to soil and human handling were present: Bacillus amyloliquefaciens, Bacillus cereus/thuringiensis, Staphylococcus epidermidis, Staphylococcus hominis ssp. novobiosepticus, and Staphylococcus intermedius. In separate experiments, new (unused) rubber picker fingers from 3 manufacturers were exposed to broiler carcass rinses, and the kinetics of bacterial attachment to finger material was determined. Turbidity of the bacterial suspensions at varying dilutions containing picker finger sections was compared hourly with controls to evaluate inhibition. New rubber finger material from the 3 manufacturers significantly inhibited bacterial growth (P < 0.05), without the aid of antibacterial additives. We improved an assay for screening disinfectants against growth of pathogens and determined the activity of 5 disinfectant compounds. Two of the compounds were most effective against Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Salmonella Enteritidis, and Escherichia coli, and one of the compounds was selected for further study with Salmonella Enteritidis. Scanning electron microscopy confirmed the levels of Salmonella Enteritidis before and after treatment. The most effective compound was nontoxic and completely cleared Salmonella Enteritidis contamination from the rubber picker finger surface.