Crystallization and Preliminary X-Ray Study of Agkistrodon halys blomhoffii Phospholipase A2 Complexed with a Specific Inhibitor

Phospholipase A2 from the venom of Agkistrodon halys blomhoffii has been crystallized as a complex with a specific inhibitor, (iS)-2-dodecanoyl-amino-3-hexanol-l-phosphoglycol. The complex crystals belong to the hexagonal space group, P6i22 (or P6622), with cell dimensions of a= 6 = 61.13 A, and c =...

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Published inJournal of biochemistry (Tokyo) Vol. 113; no. 4; pp. 411 - 412
Main Authors Tomoo, Koji, Fujiii, Shinobu, Ishida, Toshimasa, Inoue, Masaloshi, Ikeda, Kiyoshi, Samejima, Yuji, Iwama, Seiji, Katsumura, Shigeo
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.04.1993
Subjects
Animals
Crotalid Venoms - enzymology
Crystallization
Phospholipases A - antagonists & inhibitors
Phospholipases A - chemistry
Phospholipases A2
X-Rays
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Summary:Phospholipase A2 from the venom of Agkistrodon halys blomhoffii has been crystallized as a complex with a specific inhibitor, (iS)-2-dodecanoyl-amino-3-hexanol-l-phosphoglycol. The complex crystals belong to the hexagonal space group, P6i22 (or P6622), with cell dimensions of a= 6 = 61.13 A, and c = 173.15A. The diffraction extends to at least 2.3 A resolution.
Bibliography:istex:97B95EDBC9D2C0E214E21324E110BB3CA2716778
1To whom correspondence should be addressed.
ark:/67375/HXZ-XV1CNTMN-B
ArticleID:113.4.411
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a124059