Immunohistochemical localization of progesterone receptor in human decidua of early pregnancy

• Published in: Human reproduction (Oxford) Vol. 7; no. 1; p. 123
• Main Authors: Wang, J D, Fu, Y, Shi, W L, Zhu, P D, Cheng, J, Qiao, G M, Wang, Y Q, Greene, G L
• Format: Journal Article
• Language: English
• Published: England 01.01.1992
• Subjects: Adult; Antibodies, Monoclonal; Cell Nucleus - metabolism; Decidua - metabolism; Endothelium, Vascular - metabolism; Female; Humans; Immunohistochemistry; Muscle, Smooth, Vascular - metabolism; Myometrium - metabolism; Pregnancy; Pregnancy Trimester, First - metabolism; Receptors, Progesterone - analysis
• ISSN: 0268-1161
• DOI: 10.1093/oxfordjournals.humrep.a137545

Rat monoclonal antibodies to human progesterone receptor were used for immunolocalization studies in human decidua of early pregnancy. Frozen sections of 42 specimens of decidua were stained by the peroxidase-antiperoxidase method (PAP). Progesterone receptor was localized exclusively in the nuclei of decidual and myometrial cells with no specific staining in the cytoplasm. In the decidualized endometrium, stroma were always positively stained. Smooth muscle, pericyte and endothelial cells of blood vessels were extensively stained. Glandular epithelia showed variation in staining, which was positive in the basal but very weak or negative in the superficial layer of the decidua. No specific staining could be detected in the control sections. Of special interest was the positive staining of the endothelium of decidual blood vessels, a finding which has not been reported previously. The cells of the inner lining of vessels that stained with the antiprogesterone receptor antibodies were also Factor VIII positive, thus confirming the endothelial nature of these cells. It is concluded from these results that endothelial cells from human first trimester decidua express progesterone receptors.