Neurite outgrowth and cell cycle kinetic changes induced by cis-diamminedichloroplatinum II and retinoic acid in a human neuroblastoma cell line

The aim of this study was to analyze by flow cytometry the effect of cis-diamminedichloroplatinum II (CDDP) and retinoic acid (RA) on the cell cycle of a neuroblastoma cell line (SK-N-BE (2)C NB) and to correlate the kinetic data with cell morphology. CDDP at 1 μg/ml induced a dramatic G2 + M cell c...

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Bibliographic Details
Published inCancer letters Vol. 52; no. 2; pp. 101 - 106
Main Authors Di Martino, D., Avignolo, C., Marsano, B., Di Vinci, A., Cara, A., Giaretti, W., Tonini, G.P.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 16.07.1990
Elsevier
Subjects
Antineoplastic agents
Biological and medical sciences
Cell Cycle - drug effects
Cell Differentiation - drug effects
cisplatin
Cisplatin - pharmacology
differentiation
DNA, Neoplasm - analysis
Flow Cytometry
General aspects
Humans
In Vitro Techniques
Medical sciences
neuroblastoma
Neuroblastoma - pathology
Neurons - cytology
Pharmacology. Drug treatments
retinoic acid
Tretinoin - pharmacology
Tumor Cells, Cultured
neuroblastoma
retinoic acid
flow cytometry
differentiation
cisplatin
Antineoplastic agent
Human
Flow cytometry
Cell cycle
Kinetics
Neuroblastoma
Retinoids
Cell differentiation
In vitro
Retinoic acid
Tumor cell
Platinum II Complexes
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Summary:The aim of this study was to analyze by flow cytometry the effect of cis-diamminedichloroplatinum II (CDDP) and retinoic acid (RA) on the cell cycle of a neuroblastoma cell line (SK-N-BE (2)C NB) and to correlate the kinetic data with cell morphology. CDDP at 1 μg/ml induced a dramatic G2 + M cell cycle phases block (nearly 200% increase with respect to control) 2 days after treatment. The G2 + M block was spontaneously reversed starting from the 4th day. The cells treated with 10 μM RA were, instead, induced to irreversibly enter the G0 + G1 phase of the cell cycle (nearly 20% increase with respect to control) 48 h after treatment. Neurite-like structures were observed for both CDDP and RA treated cells. These data suggest different cell cycle dependent molecular mechanisms and different degrees of differentiation during CDDP or RA treatment of NB cells.
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content type line 23
ISSN:0304-3835
1872-7980
DOI:10.1016/0304-3835(90)90251-R