Survey of mycotoxins in commonly consumed Korean grain products using an LC-MS/MS multimycotoxin method in combination with immunoaffinity clean-up

• Published in: Food science and biotechnology Vol. 24; no. 4; pp. 1193 - 1199
• Main Authors: Jung, So-young, Choe, Bu-Chuhl, Choi, Eun-Jung, Jeong, Hee-Jeong, Hwang, Young-Sook, Shin, Gi-Young, Kim, Jung-Hun
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.08.2015; 한국식품과학회
• Subjects: aflatoxins; Chemistry; Chemistry and Materials Science; deoxynivalenol; Food Science; fumonisins; grain products; Korean Peninsula; markets; Nutrition; ochratoxins; quantitative analysis; Research Article; surveys; zearalenone; 식품과학; Korean Peninsula; mycotoxin; LC-MS/MS; immunoaffinity column
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-015-0153-5

A total of 47 powder of roasted grain ( sunsik ) samples were collected from markets in Seoul, Korea for a survey of mycotoxin contaminants. A multitoxin immunoaffinity column was used to clean-up extracts for all mycotoxins. An LC-MS/MS method was used to perform simultaneous quantitative determination of aflatoxins (B1, B2, G1, and G2), deoxynivalenol, fumonisin (B1, B2), ochratoxin, zearalenone, T-2, and HT-2 in sunsik samples. Limits of detetction (LOD) and quantitation (LOQ) for mycotoxins ranged 0.1–6.1 and 0.3–18.4 µg/kg, respectively. Zearalenone was detected in all sunsik samples, deoxynivalenol was detected in 38.3% of sunsik samples, ranging from 0.63 to 29.3 µg/kg and not detected (ND)-48.3 µg/kg, respectively. The daily intake values of mycotoxins from sunsik for the p95 quartile for Korean consumers were 0.42% (DON), 0.12% (FBs), 0.42% (OTA), and 1.87% (ZEA) of TDI.