Effect of secretin on pancreatic juice proteins in caerulein-induced acute pancreatitis in the rat

• Published in: Peptides (New York, N.Y. : 1980) Vol. 10; no. 2; pp. 255 - 260
• Main Authors: Antonio Manso, Manuel, De Dios, Isabel, San Roman, Jose Ignacio, Calvo, Jose Julian, Lopez, Maria Abdona
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.1989; Elsevier Science
• Subjects: Acute Disease; Acute pancreatitis; Amylases - metabolism; Animals; Biological and medical sciences; Caerulein; Ceruletide; Gastroenterology. Liver. Pancreas. Abdomen; Isoelectric Focusing; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Molecular Weight; Other diseases. Semiology; Pancreatic juice; Pancreatic Juice - drug effects; Pancreatic Juice - enzymology; Pancreatic Juice - metabolism; Pancreatitis - chemically induced; Pancreatitis - physiopathology; Proteins - isolation & purification; Proteins - metabolism; Rat; Rats; Rats, Inbred Strains; Reference Values; Secretin; Secretin - pharmacology; Secretin; Acute pancreatitis; Caerulein; Rat; Pancreatic juice; Biochemical analysis; Acute; Rodentia; Pancreatitis; Cerulein; Secretine; Vertebrata; Experimental disease; Mammalia; Enzymatic activity; Animal; Digestive diseases
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/0196-9781(89)90027-2

Nine hours after the start of treatment with caerulein in rats, an increase in the weight of the pancreas and an increase in serum amylase levels were observed. Likewise, a significant increase in endogenous secretin occurred in rats with acute pancreatitis. A dramatic reduction in the secretion of total protein and amylase was also observed. A partial recovery of this latter effect was achieved after an infusion of high doses of secretin. Under our experimental conditions, the volume of secretion did not vary in caerulein-treated rats with respect to controls, either in resting conditions or under secretin stimulation, which indicates that the ductular cells were not significantly affected. Isoelectrofocusing (IEF) and crossed-immunoelectrophoresis (CIE) studies revealed important alterations in the proteins of the pancreatic juice of rats with caerulein-induced acute pancreatitis. Trypsinogen appeared to be particularly affected, showing an increase in the T 2 acidic form with an IEP of 4.4 and a decrease in the basic form T 3 with an IEP of 8.0, which splits in other forms with a clear antigenic community. A hydrolase was also observed with an IEP of 6.2. In this sense, secretin administration may also be said to induce a significant improvement in established acute pancreatitis, since it tended to normalize the structure and proportion of the proteins secreted.