Investigation of TLR1-9 genes and miR-155 expression in dogs infected with canine distemper

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 79; p. 101711
• Main Authors: Daldaban, Fadime, Karaca Bekdik, İlknur, Aslan, Öznur, Akyüz, Bilal, Akçay, Aytaç, Arslan, Korhan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2021; Elsevier Science Ltd
• Subjects: Animals; Canine distemper; Canine Distemper Virus; Correlation coefficient; Correlation coefficients; Distemper - genetics; Distemper Virus, Canine - genetics; Dog; Dog Diseases - genetics; Dogs; Erythrocytes; Gene expression; Genes; Hematocrit; Hemoglobin; Leukocytes (neutrophilic); miR-155; miRNA; Monocytes; Polymerase chain reaction; Pretreatment; Real-Time Polymerase Chain Reaction - veterinary; Ribonucleic acid; RNA; Statistical analysis; TLR; TLR1 protein; TLR2 protein; TLR3 protein; TLR4 protein; TLR5 protein; TLR9 protein; Toll-Like Receptor 1; Toll-like receptors; TLR; Gene expression; Canine Distemper Virus; Dog; miR-155
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2021.101711

This study aimed to determine the relationship of toll-like receptor (TLR) 1-9 genes and microRNA (miR) -155 expression levels with hematologic parameters in dogs diagnosed with canine distemper. In the study, two groups were used pre-treatment and post-treatment. Infected dogs were diagnosed with canine distemper with the help of a rapid test kit and Real Time-Polymerase Chain Reaction (RT-PCR). Based on the correlation coefficients between the expression levels of the genes examined within the scope of the study and hematologic values, a positive correlation was found between the TLR2 gene and the monocyte (MON) value and between the TLR4 gene and the platelet (PLT) value in the pre-treatment group. A strong positive correlation was identified between TLR3 and TLR9 genes and erythrocyte (RBC) and hemoglobin (HGB) values; between TLR5 gene and RBC, HGB and hematocrit (HCT) values and between TLR9 gene and RBC and HGB values in the post-treatment group, on the other hand, a positive correlation was found between TLR1 gene and MON and neutrophil (GRAN) values; between TLR3 gene and HCT value and between TLR9 gene and MON and HCT values. The study concluded that miR-155 and TLR8 gene were upregulated at a statistically significant level (P < 0.05) Post-treatment in dogs infected with canine distemper and there was a positive correlation between the upregulation of miR-155 and the upregulation of TLR8 in the same period. This result suggests that the upregulated miR-155 expression post-treatment increased TLR8 gene expression. In the light of these findings, it miR-155 may have the potential to be used in clinical practice in the treatment or prognosis of dogs infected with canine distemper. •Canine distemper disease is a disease with the potential to be seen in puppies even if vaccinated.•It is important to reveal the relationship between hematological parameters and gene expression in order to establish clinical treatment protocols.•Pre and post treatment of distemper disease; changes in the expression levels of TLR8 and miR-155 demonstrate the potential in clinical practice.