A correlation of results measured by cyclic voltammogram and impedance spectroscopy in glucose oxidase based biocatalysts

• Published in: The Korean journal of chemical engineering Vol. 34; no. 11; pp. 3009 - 3016
• Main Authors: Christwardana, Marcelinus, Chung, Yongjin, Kwon, Yongchai
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2017; Springer Nature B.V; 한국화학공학회
• Subjects: Biotechnology; Carbon nanotubes; Catalysis; Catalysts; Charge transfer; Chemistry; Chemistry and Materials Science; Correlation analysis; Electrochemical impedance spectroscopy; Electrolytic cells; Electronic; Glucose; Glucose oxidase; Industrial Chemistry/Chemical Engineering; Inorganic; Materials (Organic; Materials Science; Nanotubes; Nyquist plots; Polyethyleneimine; Qualitative analysis; Reactivity; Spectrum analysis; Thin Films; 화학공학; Glucose Oxidase; Cyclic Voltammogram; Enzymatic Biofuel Cell; Charge Transfer Resistance; Flavin Adenine Dinucleotide Redox Reactivity
• ISSN: 0256-1115; 1975-7220
• DOI: 10.1007/s11814-017-0213-z

A new biocatalyst consisting of glucose oxidase (GOx) and polyethylenimine (PEI) immobilized on carbon nanotube (CNT) (CNT/PEI/GOx) was developed, while cyclic voltammogram (CV) behaviors of several related catalysts including the CNT/PEI/GOx were analyzed in terms of charge transfer resistances (R ct s) obtained by measuring Nyquist plots using electrochemical impedance spectroscopy (EIS). A qualitative correlation between the flavin adenine dinucleotide (FAD) redox reactivity measured by the CV and R ct was established. As factors affecting both the FAD reactivity and R ct , concentrations of GOx, glucose, and phosphate buffer solution, electrolyte pH and ambient condition were considered and evaluations of the catalysts using the CV curves and Nyquist plots confirmed that a pattern in the FAD reactivity was closely linked to that in the R ct , implying that FAD reactivities of the catalysts are predicted by the measurements of their R ct s. Even regarding performance of the enzymatic biofeul cells (EBCs) using the reacted catalysts, a pattern of the R ct s is compatible with that in the maximum power densities (MPDs) of the EBCs.