Engineered telomeres in transgenic Xenopus laevis

The expanding roles of telomeres in epigenetic gene regulation, nuclear organization, and human disease have necessitated the establishment of model organisms in which to study telomere function under normal developmental conditions. We present an efficient system for generating numerous vertebrate...

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Bibliographic Details
Published inTransgenic research Vol. 16; no. 3; pp. 377 - 384
Main Authors WUEBBLES, Ryan, JONES, Peter L
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.06.2007
Springer Nature B.V
Subjects
Animals
Animals, Genetically Modified - genetics
Animals, Genetically Modified - growth & development
Biological and medical sciences
Biotechnology
Chromosomes
DNA - analysis
Epigenesis, Genetic
Epigenetics
Fundamental and applied biological sciences. Psychology
Gene regulation
Genetic Engineering
Genetic technics
Genetics
In Situ Hybridization, Fluorescence
Methods. Procedures. Technologies
Models, Animal
Telomerase
Telomere - chemistry
Telomere - genetics
Telomere - physiology
Telomeres
Transgenic animals
Transgenic animals and transgenic plants
Xenopus laevis
Xenopus laevis - genetics
Xenopus laevis - growth & development
Zygotes
Vertebrata
Transgenic . Xenopus laevis
Xenopus laevis
Amphibia
Salientia
Telomere
Transgenesis
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Summary:The expanding roles of telomeres in epigenetic gene regulation, nuclear organization, and human disease have necessitated the establishment of model organisms in which to study telomere function under normal developmental conditions. We present an efficient system for generating numerous vertebrate animals containing engineered telomeres using a Xenopus laevis transgenesis technique. Our results indicate Xenopus zygotes efficiently recognize telomeric repeats at chromosome break points and form telomeric complexes thus generating a new telomere. The resulting transgenic animals progress through normal development and successfully metamorphose into froglets despite the chromosome breakage. Overall, this presents an efficient mechanism for generating engineered telomeres in a vertebrate system and provides an opportunity to investigate epigenetic aspects of telomere function during normal vertebrate development.
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ISSN:0962-8819
1573-9368
DOI:10.1007/s11248-007-9076-0