Nicotine evoked efferent transmitter release onto immature cochlear inner hair cells

• Published in: Journal of neurophysiology Vol. 124; no. 5; pp. 1377 - 1387
• Main Authors: Zhang, Y., Glowatzki, E., Roux, I., Fuchs, P. A.
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.11.2020
• Series: Auditory and Vestibular Efferents
• Subjects: Animals; Cells, Cultured; Female; Hair Cells, Auditory, Inner - drug effects; Hair Cells, Auditory, Inner - physiology; Male; Membrane Potentials - drug effects; Mice, Inbred C57BL; Neurons, Efferent - drug effects; Neurons, Efferent - physiology; Nicotine - administration & dosage; Receptors, Nicotinic - physiology; cochlea; efferent; nicotinic; presynaptic; hair cell
• ISSN: 0022-3077; 1522-1598; 1522-1598
• DOI: 10.1152/jn.00097.2020

Sensory organs exhibit spontaneous activity before the onset of response to external stimuli. Such activity in the cochlea is subject to modulation by cholinergic efferent neurons that directly inhibit sensory hair cells (inner hair cells). Those efferent neurons are themselves subject to various modulatory mechanisms. One such mechanism is positive feedback by released acetylcholine onto presynaptic nicotinic acetylcholine receptors causing further release of acetylcholine. Olivocochlear neurons make temporary cholinergic synapses on inner hair cells of the rodent cochlea in the first 2 to 3 wk after birth. Repetitive stimulation of these efferent neurons causes facilitation of evoked release and increased spontaneous release that continues for seconds to minutes. Presynaptic nicotinic acetylcholine receptors (nAChRs) are known to modulate neurotransmitter release from brain neurons. The present study explores the hypothesis that presynaptic nAChRs help to increase spontaneous release from efferent terminals on cochlear hair cells. Direct application of nicotine (which does not activate the hair cells’ α9α10-containing nAChRs) produces sustained efferent transmitter release, implicating presynaptic nAChRs in this response. The effect of nicotine was reduced by application of ryanodine that reduces release of calcium from intraterminal stores. NEW & NOTEWORTHY Sensory organs exhibit spontaneous activity before the onset of response to external stimuli. Such activity in the cochlea is subject to modulation by cholinergic efferent neurons that directly inhibit sensory hair cells (inner hair cells). Those efferent neurons are themselves subject to various modulatory mechanisms. One such mechanism is positive feedback by released acetylcholine onto presynaptic nicotinic acetylcholine receptors causing further release of acetylcholine.