Development of a glucose enzyme fuel cell based on thin film electrode using biocatalysts

• Published in: Biotechnology and bioprocess engineering Vol. 29; no. 3; pp. 529 - 542
• Main Authors: Kim, Dong Sup, Yang, Xiaoguang, Sobhan, Abdus, Park, Chulhwan, Kim, Seung Wook, Lee, Jinyoung
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.06.2024; Springer Nature B.V; 한국생물공학회
• Subjects: Atomic force microscopy; Biocatalysts; Biotechnology; Chemistry; Chemistry and Materials Science; Chitosan; Clean energy; electric potential difference; Electrodes; electron microscopy; Electron transfer; Electronic implants; Enzymes; Field emission; films (materials); Fourier analysis; Fourier transform infrared spectroscopy; Fourier transforms; Fuel cells; Fuel technology; fuels; Glucose; Glucose oxidase; Green energy; Human body; humans; Immobilization; immobilized enzymes; Industrial and Production Engineering; Industry 4.0; Infrared analysis; Infrared spectroscopy; insulin; Microscopy; Neural prostheses; Power supply; renewable energy sources; Research Paper; Scanning electron microscopy; Thin films; Transplants & implants; 생물공학; Thin film electrode; Environmental enzyme; Electrochemical oxidation; Enzymatic fuel cell
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-024-00065-x

Artificial transplantation of the human body, which requires high technology, has been an attractive issue in the 4th industrial revolution era. The artificial equipment for human applications could contain a small-scale power supply. Enzyme fuel cells (EFCs) that generate green energy are being researched for use as the power supply for pacemakers, insulin pump, and retinal implant in human body. This study focused on an (EFC) using thin film electrodes-based on enzyme immobilization technology. The performance of this EFC was improved by enzyme immobilization and electron transfer. To improve the electron transfer, the GO/Co/chitosan composite was modified on the surface of thin film electrode. The properties of this modified surface of thin film electrode were confirmed by analysis of field emission gun scanning electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The performance of the designed EFC was optimized with immobilized redox enzyme on the modified electrode. The highest power density and voltage are determined as 441.48 µW/cm 2 and − 0.443 V by thin film electrode, respectively. The optimum conditions of the EFC were 0.1 M D-glucose, 0.1 g/L glucose oxidase, pH 7.0, and reaction time of 4 h for both two types of thin film-electrodes.