Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats

• Published in: In vivo (Athens) Vol. 31; no. 1; pp. 45 - 50
• Main Authors: Søe, Niels H, Jensen, Nina Vendel, Jensen, Asger Lundorff, Koch, Janne, Poulsen, Steen Seier, Pier, Gerald B, Johansen, Helle Krogh
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 05.01.2017
• Subjects: Animals; Biomarkers - analysis; Immunization - methods; Male; Osteomyelitis - immunology; Osteomyelitis - microbiology; Osteomyelitis - prevention & control; Periprosthetic Fractures - immunology; Periprosthetic Fractures - microbiology; Periprosthetic Fractures - prevention & control; Rabbits; Rats; Rats, Sprague-Dawley; Staphylococcal Infections - immunology; Staphylococcal Infections - microbiology; Staphylococcal Infections - prevention & control; Staphylococcus aureus - immunology; Staphylococcus aureus - pathogenicity; Osteomyelitis; ica+ strain; implant-related infection Staphylococcus aureus; vaccine; ica− strain; immunization
• ISSN: 1791-7549; 0258-851X; 1791-7549
• DOI: 10.21873/invivo.11023

Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization. Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10 S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus. Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica ) but not ica S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica or ica S. aureus, suggesting a lack of specificity in this antiserum.