Amplification of HIV-1 Provirus from Cerebrospinal Fluid and Its Correlation with Neurologic Disease

• Published in: The Journal of infectious diseases Vol. 161; no. 6; pp. 1068 - 1072
• Main Authors: Shaunak, Sunil, Albright, Robert E., Klotman, Mary E., Henry, Stanley C., Bartlett, John A., Hamilton, John D.
• Format: Journal Article
• Language: English
• Published: United States The University of Chicago Press 01.06.1990; University of Chicago Press
• Subjects: Adult; AIDS; AIDS Dementia Complex - microbiology; AIDS/HIV; Base Sequence; Central nervous system; Cerebrospinal fluid; Cerebrospinal Fluid - microbiology; Child; DNA, Viral - analysis; Female; Follow-Up Studies; Genes, env; Genes, gag; HIV; HIV 1; HIV Infections - complications; HIV Infections - microbiology; HIV-1 - genetics; HIV-1 - isolation & purification; Humans; Lymphocytes; Magnetic Resonance Imaging; Major Articles; Male; Molecular Sequence Data; Nervous system diseases; Nervous System Diseases - complications; Nervous System Diseases - microbiology; Neurologic manifestations; Oligonucleotide Probes; Polymerase Chain Reaction; Proviruses; Proviruses - genetics; Proviruses - isolation & purification; Retrospective Studies; Tomography, X-Ray Computed
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/161.6.1068

The polymerase chain reaction (PCR) wasused to detect human immunodeficiency virus type 1 (HIV-l) proviral sequences (gag and env) in nucleated cells from the cerebrospinal fluid (CSF) of 31 HIV-l-positive patients, and the results were compared with clinical and radiologic evidence of neurologic disease. Provirus was detected in 21 patients, of whom 20 had neurologic abnormalities. Provirus was not detected in another 6, all ofwhom were neurologically normal. No neurologic disease has developed in 4 of these 6 patients for whom 12.8 months of follow-up is available. PCR of CSF nucleated cells from HIV-positive patients provides early, rapid, direct evidence of neurologic involvement.