The cesium-induced delay in myoblast membrane fusion is accompanied by changes in isolated membrane lipids

• Published in: Biochimica et biophysica acta Vol. 1023; no. 2; pp. 298 - 304
• Main Authors: SANTINI, M. T, INDOVINA, P. L, CANTAFORA, A, BLOTTA, I
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 13.04.1990; North-Holland
• Subjects: Animals; Biological and medical sciences; Cell differentiation, maturation, development, hematopoiesis; Cell Membrane - drug effects; Cell Membrane - physiology; Cell physiology; Cells, Cultured; Cesium - pharmacology; Chick Embryo; Cholesterol - metabolism; Electric Conductivity; Fundamental and applied biological sciences. Psychology; Lipid Bilayers - metabolism; Membrane Fusion; Membrane Lipids - metabolism; Molecular and cellular biology; Muscles - drug effects; Muscles - ultrastructure; Phosphatidic Acids - metabolism; Phosphatidylcholines - metabolism; Phosphatidylethanolamines - metabolism; Phosphatidylinositols - metabolism; Second Messenger Systems; Cell culture; Phospholipid; Chromatography; Vertebrata; Enzymatic activity; Plasma membrane; Development; Chemical composition; Muscle; Chicken; Membrane potential; Aves; Quantitative analysis
• ISSN: 0006-3002; 1878-2434
• DOI: 10.1016/0005-2736(90)90426-O

We have recently demonstrated that cesium ions delay the sharp decrease in both membrane conductivity and membrane permittivity of chick embryo myoblasts seen at fusion (Santini, M.T., Bonincontro, A., Cametti, C. and Indovina, P.L. (1988) Biochim. Biophys. Acta 945, 56-64). Analysis of the conductivity dispersion data (obtained in the radiowave frequency range) indicated that cesium delays fusion by about 30 h. We suggested that cesium is affecting both active ionic transport by blocking potassium channels as well as interfering with membrane lipid and/or protein charges. In the present study, we have investigated both the possible role of membrane lipids in myoblast fusion and the possible effects of cesium on these lipids. Our data indicate that lipid changes do occur in the isolated myoblast plasma membrane of controls during myogenic differentiation especially prior to fusion and that in cesium cultures these variations do not occur. These variations are in accordance with current membrane fusion theory. Specifically, there is a decrease in bilayer-stabilizing lipids (phosphatidylcholine) and an increase in bilayer-destabilizing ones (phosphatidylethanolamine and phosphatidic acid) and cholesterol during the fusion process. In addition, although slight, during fusion there appears to be a decrease in phosphatidylinositol which is believed to be involved in the inositol phosphate second messenger system. In cesium cultures, in which fusion is greatly delayed, the same lipid changes do not take place and those that are observed seem to reflect the fusion delay.