G and P genotype profiles of rotavirus A field strains circulating in beef and dairy cattle herds in Brazil, 2006–2015

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 64; pp. 90 - 98
• Main Authors: da Silva Medeiros, Thais Neris, Lorenzetti, Elis, Alfieri, Alice Fernandes, Alfieri, Amauri Alcindo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2019; Elsevier Science Ltd
• Subjects: Beef; Calves; Cattle; Dairy cattle; Diarrhea; Gene sequencing; Genotype & phenotype; Genotype G; Genotype P; Genotypes; Lineages; Nucleotides; Polymerase chain reaction; Rotavirus; RVA; Strains (organisms); Viruses; VP4 gene; VP7 gene; Brazil; Lineages; Genotype G; Calves; Diarrhea; Genotype P; RVA; VP7 gene; VP4 gene
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2019.03.002

•The G and P genotype diversity in Brazilian strains of bovine RVA is very low.•RVA genotype G6P[5] was more prevalent in Brazilian beef cattle herds.•G10P[11] and G6P[11] RVA strains occur more frequently in dairy cattle herds.•Bovine RVA strains genotype G10 lineage III were detected in Brazil.•First detection of P[5]-II and P[11]-VII lineages in Brazilian bovine RVA strains. The aim of this retrospective study was to use RT-PCR and nucleotide sequencing analysis to determine the G (VP7 gene) and P (VP4 gene) genotypes of 155 Brazilian bovine rotavirus A (RVA) wild-type strains detected in diarrheic calves from all Brazilian geographical regions from 2006 to 2015. The RVA strains evaluated belonged to the G6, G10, P[5], and P[11] genotypes. The G6P[5] genotype was prevalent (65.5%; P < 0.05) in beef, and the G10P[11] (38.4%) and G6P[11] (30.8%) genotypes were more prevalent in dairy cattle herds. The Midwest was the region with the highest number of genotyped RVA strains, where the genotypes G6, P[5], and P[11] were identified. Genotype combination G6-IV/P[5]-IX, prevalent in beef herds, and G6-III/P[11]-III or G10-IV/P[11]-III, prevalent in dairy herds, were detected. In addition, for the first time in Brazil, we detected the P[5] and P[11] genotype RVA strains that belong to lineage II and VII, respectively.