Time-resolved polarization measurements of porphyrin fluorescence in solution and in single cells

Porphyrin monomers, dimers and aggregates, which can be differentiated on the basis of their fluorescence lifetimes, are shown to possess different degrees of fluorescence polarization. This opens up new possibilities for microscopic imaging of these individual components in photosensitization and t...

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Bibliographic Details
Published inJournal of photochemistry and photobiology. B, Biology Vol. 5; no. 3-4; p. 391
Main Authors Seidlitz, H K, Schneckenburger, H, Stettmaier, K
Format Journal Article
LanguageEnglish
Published Switzerland 01.05.1990
Subjects
Animals
Cell Line
Epithelium - analysis
Fluorescence Polarization - methods
Porphyrins - analysis
Protoporphyrins - analysis
Rats
Solutions
Time Factors
Vero Cells
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Summary:Porphyrin monomers, dimers and aggregates, which can be differentiated on the basis of their fluorescence lifetimes, are shown to possess different degrees of fluorescence polarization. This opens up new possibilities for microscopic imaging of these individual components in photosensitization and tumour detection. A rough estimate of the size of the porphyrin aggregates is obtained from the data of time-resolved fluorescence anisotropy.
ISSN:1011-1344
DOI:10.1016/1011-1344(90)85053-Y