Human UDP-Galactose Translocator: Molecular Cloning of a Complementary DNA That Complements the Genetic Defect of a Mutant Cell Line Deficient in UDP-Galactose Translocator

• Published in: Journal of biochemistry (Tokyo) Vol. 120; no. 2; pp. 236 - 241
• Main Authors: Miura, Nobuhiko, Ishida, Nobuhiro, Hoshino, Masato, Yamauchi, Masatake, Hara, Takahiko, Ayusawa, Dai, Kawakita, Masao
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.08.1996
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological Transport, Active; Caenorhabditis elegans - genetics; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; CDC91; Cell Line; Cloning, Molecular; DNA, Complementary - genetics; Genetic Complementation Test; Golgi apparatus; Golgi Apparatus - metabolism; Humans; Mice; Molecular Sequence Data; Monosaccharide Transport Proteins - chemistry; Monosaccharide Transport Proteins - genetics; Monosaccharide Transport Proteins - metabolism; Mutation; Open Reading Frames; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Sequence Homology, Amino Acid; UDP-galactose; UDP-galactose translocator; Uridine Diphosphate Galactose - metabolism; ZK370.7
• ISSN: 0021-924X
• DOI: 10.1093/oxfordjournals.jbchem.a021404

We have cloned a cDNA that codes for a putative human UDP-galactose translocator (UGT) protein. The cDNA contained an open reading frame of 1,179 base pairs encoding a novel protein of 393 amino acids. Introduction of the open reading frame sequence into a UGT-deficient mouse cell line, Had-1, complemented the genetic defect of the mutant, namely the inability to transport UDP-galactose from the cytosol to the Golgi lumen, as judged from the lectin-sensitivity spectrum of the transformant. To our knowledge, this is the first mammalian nucleotide-sugar translocator whose cDNA sequence has been described.