Highly sensitive glucose biosensor using new glucose oxidase based biocatalyst

• Published in: The Korean journal of chemical engineering Vol. 34; no. 11; pp. 2916 - 2921
• Main Authors: Christwardana, Marcelinus, Ji, Jungyeon, Chung, Yongjin, Kwon, Yongchai
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2017; Springer Nature B.V; 한국화학공학회
• Subjects: Ascorbic acid; Biosensors; Biotechnology; Carbon nanotubes; Catalysis; Catalysts; Catalytic activity; Chemical synthesis; Chemistry; Chemistry and Materials Science; Diabetes mellitus; Electron transport; Glucose; Glucose oxidase; Hydrophobicity; Hypoglycemia; Industrial Chemistry/Chemical Engineering; Materials Science; Nanotubes; Polyethyleneimine; Selectivity; Sensitivity analysis; Stability analysis; Uric acid; 화학공학; Glucose Oxidase; Pyrenecarboxaldehyde; Diabetes; Glucose Biosensor; Hypoglycemia
• ISSN: 0256-1115; 1975-7220
• DOI: 10.1007/s11814-017-0224-9

Glucose, which is a primary energy source of living organisms, can induce diabetes or hypoglycemia if its concentration in blood is irregular. It is therefore important to develop glucose biosensor that reads the concentration of glucose in blood precisely. In the present work, we suggest new glucose oxidase (GOx) based catalysts that can improve the sensitivity of the glucose biosensor and make glucose measurements over a wide concentration ranges possible. For synthesizing such catalysts, a composite including pyrenecarboxaldehyde (PCA) and GOx is attached to substrate including carbon nanotube (CNT) and polyethyleneimine (PEI) (CNT/PEI/[PCA/GOx]). Catalytic activity and stability of the catalyst are then evaluated. According to the investigation, the catalyst shows excellent glucose sensitivity of 47.83 μAcm −2 mM −1 , low Michaelis-Menten constant of 2.2 mM, and wide glucose concentration detection, while it has good glucose selectivity against inhibitors, such as uric acid and ascorbic acid. Also, its activity is maintained to 95.7% of its initial value even after four weeks, confirming the catalyst is stable enough. The excellence of the catalyst is attributed to hydrophobic interaction, C=N bonds, and π -hydrogen interaction among GOx, PCA and PEI/CNT. The bindings play a role in facilitating electron transport between GOx and electrode.