TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 64; pp. 31 - 39
• Main Authors: Álvarez-Estrada, Álvaro, Rodríguez-Ferri, Elías Fernando, Martínez-Martínez, Sonia, Álvarez, Belén, Fernández-Caballero, Teresa, Domínguez, Javier, Gutiérrez-Martín, Cesar Bernardo
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2019; Elsevier Science Ltd
• Subjects: Blood; CD163; CD163 antigen; Cell surface; Experimental infection; Flow cytometry; Glässer´s disease; Haemophilus parasuis; Immune response; Immune system; Leukocytes (mononuclear); Monocytes; Peripheral blood mononuclear cells; Proteins; Receptors; Sepsis; Siglec; Swine; TLR; TLR2 protein; TLR4 protein; Toll-like receptors; Sepsis; CD163; Siglec; TLR; Glässer´s disease; Haemophilus parasuis
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2019.02.001

•CD163 on blood monocytes is increased during Haemophilus parasuis-induced sepsis.•TLR2 on blood monocytes is increased during H. parasuis-induced sepsis.•Siglec-3 on blood monocytes is increased during H. parasuis-induced sepsis.•Correlation between expression of CD163 and the other two receptors were seen.•Changes seem to be due to a recruitment of monocytes from the bone marrow. TLRs, Siglecs and CD163 are cell surface receptors that play an important role in immune response and sepsis. The objective of this study was to assess changes in the expression levels of several of these receptors (TLR2, TLR4, CD163, Siglec-1, Siglec-3, Siglec-5 and Siglec-10) on the surface of peripheral blood mononuclear cells from pigs with sepsis caused by Haemophilus parasuis. Flow cytometry was employed to analyze samples from an experimental infection and from cell cultures. A significant increase in CD163, TLR2 and Siglec-3 expression during infection was seen. However, in vitro exposure of peripheral blood monocytes to bacteria or sera from infected pigs did not increase the expression of these receptors. These changes may be due to recruitment of monocytes into the blood compartment in response to H. parasuis-induced sepsis.