Serum proteomic patterns for gastric lesions as revealed by SELDI mass spectrometry

• Published in: Experimental and molecular pathology Vol. 81; no. 2; pp. 176 - 180
• Main Authors: Liang, Yong, Fang, Marong, Li, Jicheng, Liu, Chi Bo, Rudd, John A., Kung, H.F., Yew, David T.W.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.10.2006; Elsevier
• Subjects: Adenocarcinoma - blood; Adenocarcinoma - diagnosis; Biological and medical sciences; Biomarker; Biomarkers, Tumor - blood; Cancer; Case-Control Studies; Gastric adenocarcinoma; Gastritis; Gastritis - blood; Gastritis - diagnosis; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Neoplasm Invasiveness - diagnosis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Protein Array Analysis; Proteomics - methods; SELDI; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Stomach Neoplasms - blood; Stomach Neoplasms - diagnosis; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Biomarker; Gastric adenocarcinoma; SELDI; Gastritis; Cancer; Digestive system; Biological marker; Biological indicator; Malignant tumor; Stomach adenocarcinoma; Stomach cancer; Anatomic pathology; Proteomics; Digestive diseases; Serum; Lesion; Mass spectrometry; Gastric disease
• ISSN: 0014-4800; 1096-0945
• DOI: 10.1016/j.yexmp.2006.04.008

SELDI mass spectrometry was used to investigate protein expression in sera of patients with gastric cancer and gastritis compared to normal volunteers. Differences in peak morphology and intensity were observed in regions of 5910 Da, 5084 Da, 6640 Da and 8691 Da. Patients with gastric cancer exhibited an up-regulation of the 5910 Da peak and a down-regulation of the 8691 Da peak compared to the healthy volunteers; there was also some bi-partitioning and tri-partitioning at the 5084 Da peak. When comparing the sera of these cancer patients with those of gastritis, the former had an up-regulation of the 5910 Da peak and a down-regulation of the 6640 Da peak. This is the first report showing that SELDI sera analysis may be useful in the screening of gastric lesions.