The local lymph node assay being too sensitive?

The local lymph node assay (LLNA) and modifications thereof were recently recognized by the OECD as stand-alone methods for the detection of skin-sensitizing potential. However, although the validity of the LLNA was acknowledged by the ICCVAM, attention was drawn to one major problem, i.e., the poss...

Full description

Saved in:
Bibliographic Details
Published inArchives of toxicology Vol. 79; no. 12; pp. 721 - 728
Main Authors HANS-WERNER, Vohr, JURGEN, Ahr Hans
Format Journal Article
LanguageEnglish
Published Berlin Springer 01.12.2005
Springer Nature B.V
Subjects
Allergens - toxicity
Animals
Biological and medical sciences
Cell adhesion & migration
Cell Proliferation - drug effects
Dermatitis, Allergic Contact
False Positive Reactions
Female
Guinea Pigs
Irritants - toxicity
Local Lymph Node Assay
Medical sciences
Mice
Mice, Inbred Strains
Patch Tests
Predictive Value of Tests
Reproducibility of Results
Toxicology
LLNA
IMDS
Vertebrata
Mammalia
Guinea pig
Lymph node
Guinea pig assay
Toxicity
Animal
Rodentia
Sensitization
Irritation
Online AccessGet full text

Cover

More Information
Summary:The local lymph node assay (LLNA) and modifications thereof were recently recognized by the OECD as stand-alone methods for the detection of skin-sensitizing potential. However, although the validity of the LLNA was acknowledged by the ICCVAM, attention was drawn to one major problem, i.e., the possibility of false positive results caused by non-specific cell activation as a result of inflammatory processes in the skin (irritation). This is based on the fact that inflammatory processes in the skin may lead to non-specific activation of dendritic cells, cell migration and non-specific proliferation of lymph node cells. Measuring cell proliferation by radioactive or non-radioactive methods, without taking the irritating properties of test items into account, leads thus to false positive reactions. In this paper, we have compared both endpoints: (1) cell proliferation alone and (2) cell proliferation in combination with inflammatory (irritating) processes. It turned out that a considerable number of tests were "false positive" to the definition mentioned above. By excluding such false positive results the LLNA seems not to be more sensitive than relevant guinea pig assays. These various methods and results are described here.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0340-5761
1432-0738
DOI:10.1007/s00204-005-0005-9