Erythropoietin administration is associated with short-term improvement in glomerular filtration rate after ischemia-reperfusion injury
Background: Erythropoietin (EPO) is a cytokine with organ‐protective properties. We hypothesized that EPO could attenuate acute renal dysfunction and inflammation in a porcine model of ischemia–reperfusion (IR). Furthermore, we aimed to characterize the impact of EPO on systemic and renal hemodynami...
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Published in | Acta anaesthesiologica Scandinavica Vol. 55; no. 2; pp. 185 - 195 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.02.2011
Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | Background: Erythropoietin (EPO) is a cytokine with organ‐protective properties. We hypothesized that EPO could attenuate acute renal dysfunction and inflammation in a porcine model of ischemia–reperfusion (IR). Furthermore, we aimed to characterize the impact of EPO on systemic and renal hemodynamics, and renal oxygen consumption.
Methods: Twenty‐four pigs were randomly assigned to three groups: (1) EPO (5000 IU/kg) administered intravenously before IR (n=9), (2) placebo administered before IR (n=9), or (3) sham group, anesthetized and operated on only (n=6). IR was induced by clamping the left renal artery for 45 min. Hemodynamics and renal blood flow (RBF) were analyzed continuously. Glomerular filtration rate (GFR), renal oxygen consumption, and plasma cytokines (IL‐1β, IL‐6, IL‐8, IL‐10, and TNF‐α) were analyzed hourly. Renal biopsies were analyzed for cytokine content and apoptosis.
Results: GFR was higher during reperfusion in the EPO group than in the placebo group (P<0.01). No differences between the IR groups were found in hemodynamics, RBF, oxygen consumption, or renal apoptosis. The levels of TNF‐α in the plasma (P=0.036) and the levels of TNF‐α and IL‐10 in the renal cortex (P=0.04 and P=0.01, respectively) were lower in the EPO group compared with the sham group.
Conclusion: EPO attenuated the renal dysfunction as estimated as GFR. This effect was not related to changes in the hemodynamics. The immunomodulatory effects of EPO were manifested as decreased levels of TNF‐α and IL‐10 in renal biopsies and TNF‐α levels in plasma. |
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Bibliography: | istex:CF2343214CEA5E48504A6AF16D6066C9D43F6019 ArticleID:AAS2369 ark:/67375/WNG-VJDCM7ZR-Q ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0001-5172 1399-6576 |
DOI: | 10.1111/j.1399-6576.2010.02369.x |