Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes

• Published in: Nutrients Vol. 14; no. 10; p. 2158
• Main Authors: Włodarczyk, Michał, Śliżewska, Katarzyna, Barczyńska, Renata, Kapuśniak, Janusz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.05.2022; MDPI
• Subjects: Bacteria; bacterial enzymes; Bacteroides; Bifidobacterium; Bifidobacterium - metabolism; Cellulases - metabolism; Cellulases - pharmacology; Clostridium; Coculture Techniques; Colorectal cancer; Cultivation; Dextrin; Dextrins - chemistry; Dextrins - pharmacology; Diet; E coli; Enterococcus; Enzymes; Escherichia coli; Feces; Galactosidase; Glucosidase; Glucuronidase - metabolism; gut microbiota; Health promotion; Immune system; Intestine; Lactobacillus; Large intestine; Microbiota; Microorganisms; Potatoes; prebiotic; Prebiotics; Probiotics; resistant dextrin; Solanum tuberosum - chemistry; Spectrophotometry; Starch; Starch - metabolism; Strains (organisms); Work stations; α-Glucosidase; β-Galactosidase; β-Glucosidase; Poland; United States > US; bacterial enzymes; resistant dextrin; prebiotic; gut microbiota
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14102158

Preparations of resistant dextrins have become an interesting topic of research due to their properties, which bear resemblance those of prebiotics, e.g., the improvement of metabolic parameters, increased efficiency of the immune system and induction of vitamin production. The aim of this study was to investigate the effects of the resistant dextrin produced from potato starch on the growth dynamics of typical gastrointestinal microbiota and the activity of fecal enzymes in order to assess a possible exhibition of prebiotic properties. In the study, in vitro cultivation of co-cultures of Lactobacillus, Bifidobacterium, E. coli, Enterococcus, Clostridium and Bacteroides spp. was conducted on media enriched with the resistant dextrin. The CFU/mL for each strain was measured in time periods of 24, 48, 72, 96 and 168 h. Furthermore, the activities of α-glucosidase, α-galactosidase, β-glucosidase, β-galactosidase and β-glucuronidase were determined using spectrophotometric methods at a wavelength of 400 nm. The results show that the resistant dextrin can be utilized as a source of carbon for the growth of intestinal bacteria. Moreover, the results revealed that, after 168 h of cultivation, it enhances the viability of probiotic strains of Lactobacillus and Bifidobacterium spp. and decreases the growth of other intestinal strains (Clostridium, Escherichia coli, Enterococcus and Bacteroides), which is demonstrated by a high Prebiotic Index (p < 0.05). Furthermore, there was no significant change in the pH of the cultures; however, the pace of the pH decrease during the cultivation was slower in the case of culture with resistant dextrin. Furthermore, it was revealed that usage of the resistant dextrin as a medium additive noticeably lowered the activities of β-glucosidase and β-glucuronidase compared to the control (p < 0.05), whereas the activities of the other fecal enzymes were affected to a lesser degree. The resistant dextrins derived from potato starch are a suitable prebiotic candidate as they promote the growth of beneficial strains of gut bacteria and improve health markers, such as the activity of fecal enzymes. Nevertheless, additional in vivo research is necessary to further assess the suspected health-promoting properties.